Anti-PSD95 抗体 [6G6-1C9] - Synaptic Marker (ab2723)

Reviews (14)Q&A (3)References (106)

Key features and details

  • Mouse monoclonal [6G6-1C9] to PSD95 - Synaptic Marker
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker
    PSD95 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [6G6-1C9] to PSD95 - Synaptic Marker

  • 由来種

    Mouse

  • 特異性

    We do not guarantee IHC-P for mouse.

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Mouse, Rat
    交差が予測される動物種: Cow, Zebrafish

  • 免疫原

    Recombinant full length protein corresponding to Rat PSD95.

  • ポジティブ･コントロール

    • IHC-P: Rat retina tissue. WB: Rat and mouse hippocampus and forebrain tissue lysate.

  • 特記事項

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Protocols tab contains a Mouse on Mouse staining protocol with recommendations when using a mouse monoclonal antibody to stain mouse tissues and tips for reducing background.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

We do not guarantee IHC-P for mouse.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 80 kDa).

ターゲット情報

  • 機能

    Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.

  • 組織特異性

    Brain.

  • 配列類似性

    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.

  • ドメイン

    The PDZ domain 3 mediates interaction with ADR1B.
    The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.

  • 翻訳後修飾

    Palmitoylation of isoform 1 is required for targeting to postsynaptic density.

  • 細胞内局在

    Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Discs large homolog 4 antibody
    • Disks large homolog 4 antibody
    • DLG 4 antibody
    • Dlg4 antibody
    • DLG4_HUMAN antibody
    • FLJ97752 antibody
    • FLJ98574 antibody
    • Human post synaptic density protein 95 antibody
    • Post synaptic density protein 95 antibody
    • Postsynaptic density protein 95 antibody
    • PSD 95 antibody
    • PSD-95 antibody
    • PSD95 antibody
    • SAP 90 antibody
    • SAP-90 antibody
    • SAP90 antibody
    • Synapse associated protein 90 antibody
    • Synapse-associated protein 90 antibody
    • Tax interaction protein 15 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)

    IHC image of ab2723 staining in formalin-fixed, paraffin-embedded rat retinal tissue, performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2723, 2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

    Secondary-only control image is shown as insert.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
    Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
    All lanes : Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723) at 5 µg/ml

    Lane 1 : Hippocampus (Rat) Tissue Lysate
    Lane 2 : Forebrain (Rat) Tissue Lysate
    Lane 3 : Hippocampus (Mouse) Tissue Lysate
    Lane 4 : Forebrain (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 80 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa, 110 kDa, 80 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
    Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)Image courtesy of an AbReview submitted by Grant Corbett
    Lanes 1-6 : Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723) at 1/1200 dilution
    Lanes 7-12 : Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723) at 1/1200 dilution (18 hours at 4°C)

    Lanes 1-6 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
    Lanes 7-12 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Li-Cor IRDye® Donkey anti-mouse 680LT at 1/18000 dilution

    Performed under reducing conditions.

    Predicted band size: 80 kDa
    Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 5 minutes

    See Abreview

参考文献 (106)

ab2723 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2723 は 106 報の論文で使用されています。

  • Sun T  et al. Anxiolytic Effects of 8-O-Acetyl Shanzhiside Methylester on Acute and Chronic Anxiety via Inflammatory Response Inhibition and Excitatory/Inhibitory Transmission Imbalance. Neurotox Res N/A:N/A (2020). PubMed: 32367473
  • Shah BS  et al. Regulation of dendrite morphology and excitatory synapse formation by zDHHC15. J Cell Sci 132:N/A (2019). PubMed: 31189538
  • Hyvärinen T  et al. Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures. Sci Rep 9:17125 (2019). PubMed: 31748598
  • Peterson AR & Binder DK Regulation of Synaptosomal GLT-1 and GLAST during Epileptogenesis. Neuroscience 411:185-201 (2019). PubMed: 31158434
  • Merino-Serrais P  et al. 27-Hydroxycholesterol Induces Aberrant Morphology and Synaptic Dysfunction in Hippocampal Neurons. Cereb Cortex 29:429-446 (2019). PubMed: 30395175
View all Publications for this product

レビューと Q&A

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1-10 of 17 Abreviews or Q&A

Western blot abreview for Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Frontal cortex)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Frontal cortex
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
Read More

Dr. Alexandre Magno

Verified customer

投稿 Jun 26 2019

Western blot abreview for Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse brain primary culture)
Gel Running Conditions
Reduced Denaturing (Reduced Denaturing (NuPAGE 4-12% Bis-Tris precast protein gel))
Loading amount
400000 cells
Specification
Mouse brain primary culture
Blocking step
Li-Cor Odyssey blocking buffer as blocking agent as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 100% · Temperature: 28°C
Read More

Abcam user community

Verified customer

投稿 Jan 10 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (primary hippocampal neurons)
Permeabilization
Yes - 0.2% TritonX 100
Specification
primary hippocampal neurons
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More

Dr. Bhavin Shah

Verified customer

投稿 Apr 09 2018

Immunohistochemistry (Frozen sections) abreview for Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Zebrafish Tissue sections (retina, outer plexiform layer)
Permeabilization
Yes - triton X
Specification
retina, outer plexiform layer
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

Dr. Ryan Macdonald

Verified customer

投稿 Oct 02 2014

Western blot abreview for Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus and Liver)
Gel Running Conditions
Reduced Denaturing (8% in TGS)
Loading amount
30 µg
Specification
Hippocampus and Liver
Blocking step
Li-Cor Block Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Read More

Grant Corbett

Verified customer

投稿 Jul 17 2014

Immunoprecipitation abreview for Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (mouse brain tissue)
Total protein in input
200 µg
Immuno-precipitation step
Protein A/G
Specification
mouse brain tissue
Read More

Abcam user community

Verified customer

投稿 Sep 19 2013

Western blot abreview for Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse hippocampus and cerebellum)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
mouse hippocampus and cerebellum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Sep 19 2013

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker

 Good
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain
Fixative
Paraformaldehyde
Read More

Dr. Sophie Pezet

Verified customer

投稿 Jul 04 2012

Question

Will PSD95 expression be altered if toxicity is increased in the cells?

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Answer

We do not know how PSD95 expression would be affected if toxicity is increased in cells. We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests.

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Question

Inquiry: We are looking for a loading control for our western blots that is at a minimum 75kDa in size (preferably over 100kDa). Is there anything you could recommend? Our cell type is mouse neurons.

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Answer

Thank you for contacting Abcam.

In mouse neurons an alternative to the smaller housekeeping proteins such as beta-actin, or GAPDH commonly used as loading control would be to use synaptic markers such as PSD95 or Piccolo.

In western blot I would recommend use of anti-PSD95 antibodieshttps://www.abcam.com/PSD95-antibody-Synaptic-Marker-ab18258.htmlorhttps://www.abcam.com/PSD95-antibody-6G6-1C9-ab2723.htmlwhich will give you band above your threshold size at 95kDA.

Should you want bands with an even higher molecular weight Piccolo has a predicted size at 540kDA, our producthttps://www.abcam.com/Piccolo-antibody-Synaptic-Marker-ab20664.htmlis, like the above PSD95 antibodies, guaranteed to work on mouse samples in Western blot.



I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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1-10 of 17 Abreviews or Q&A

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