Anti-PSD93 抗体 (ab12097)
Key features and details
- Goat polyclonal to PSD93
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PSD93 antibody
PSD93 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to PSD93 -
由来種
Goat -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Mouse PSD93 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab12392) -
ポジティブ･コントロール
- This antibody gave a positive result when used in the following formaldehyde fixed cell lines: SKNSH
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特記事項
PSD93 is part of the PSD-95/SAP90 family containing a modular structure with three PDZ-, one SH3- and a guanylate kinase-like domain. PSD93 is expressed in discrete neuronal populations as well as in specific non-neuronal cells. PSD93, like PSD-95, binds to NMDA receptors and to the neuronal nitric oxide synthase (NOS). PSD93 and PSD95 can heteromultimerize with each other and are recruited into the same NMDA receptor and K+ channel clusters. PSD93, however, is unique among PSD95/SAP90 family members in its expression in Purkinje neuron cell bodies and dendrites.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.025% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
PSD93 is part of the PSD-95/SAP90 family containing a modular structure with three PDZ-, one SH3- and a guanylate kinase-like domain. PSD93 is expressed in discrete neuronal populations as well as in specific non-neuronal cells. PSD93, like PSD-95, binds to NMDA receptors and to the neuronal nitric oxide synthase (NOS). PSD93 and PSD95 can heteromultimerize with each other and are recruited into the same NMDA receptor and K+ channel clusters. PSD93, however, is unique among PSD95/SAP90 family members in its expression in Purkinje neuron cell bodies and dendrites. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12097の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 10 µg/ml.
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| IHC-P | (2) |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 0.3 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 10 µg/ml. |
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IHC-P
Use at an assay dependent concentration. |
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WB
Use a concentration of 0.3 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). |
ターゲット情報
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機能
Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses. -
配列類似性
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
ドメイン
An N-terminally truncated L27 domain is predicted in isoform 2 at positions 1 through 27. -
翻訳後修飾
Palmitoylation of isoform 1 is not required for targeting to postsynaptic density. -
細胞内局在
Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Membrane. Cell projection, axon. Concentrated in soma and postsynaptic density of a subset of neurons. - Information by UniProt
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参照データベース
- Entrez Gene: 1740 Human
- Entrez Gene: 23859 Mouse
- Entrez Gene: 64053 Rat
- Omim: 603583 Human
- SwissProt: Q15700 Human
- SwissProt: Q91XM9 Mouse
- SwissProt: Q63622 Rat
- Unigene: 367656 Human
see all -
別名
- Channel associated protein of synapse 110 antibody
- Channel associated protein of synapses 110kD antibody
- Channel-associated protein of synapse-110 antibody
see all
画像
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Western blot - Anti-PSD93 antibody (ab12097)This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
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Immunocytochemistry/ Immunofluorescence - Anti-PSD93 antibody (ab12097)ICC/IF image of ab12097 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab12097 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 donkey anti- goat (ab150133) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD93 antibody (ab12097)ab12097 PSD staining (10µ µ -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD93 antibody (ab12097)This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdomab12097 PSD staining (10µ g/ml) on mouse brain (5µ m sagittal section on slide; 4% PFA perfused & paraffin embedded). Granular staining observed as expected in [A] cerebellum and [B] hippocampus with ab12097. Blue=haemotoxylin nuclear counter-stain. Images taken with X10 objective.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab12097 は論文での使用が確認できていません。
