Anti-PRPF3 抗体 (ab272596)
Key features and details
- Rabbit polyclonal to PRPF3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PRPF3 antibody -
製品の詳細
Rabbit polyclonal to PRPF3 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow, Orangutan -
免疫原
Recombinant fragment corresponding to Human PRPF3 aa 350-500.
Database link: O43395 -
ポジティブ・コントロール
- WB: HEK-293T cells. IHC-P: Human stomach tissue. ICC/IF: human cell line A431.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab272596の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. -
組織特異性
Highly expressed in retina, liver, kidney and blood. Detected at lower levels in heart and brain. -
関連疾患
Retinitis pigmentosa 18 -
配列類似性
Contains 1 PWI domain. -
細胞内局在
Nucleus speckle. Colocalizes with spliceosomal snRNPs. - Information by UniProt
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参照データベース
- Entrez Gene: 510322 Cow
- Entrez Gene: 9129 Human
- Entrez Gene: 70767 Mouse
- Entrez Gene: 100440626 Orangutan
- Omim: 607301 Human
- SwissProt: Q2KIA6 Cow
- SwissProt: O43395 Human
- SwissProt: Q922U1 Mouse
see all -
別名
- hPrp3 antibody
- HPRP3P antibody
- Pre mRNA splicing factor 3 antibody
see all
画像
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All lanes : Anti-PRPF3 antibody (ab272596) at 0.4 µg/ml
Lane 1 : Control (vector only transfected HEK-293T lysate)
Lane 2 : PRPF3 over-expression lysate in mammalian HEK-293T cells -
Paraffin-embedded formalin-fixed Human stomach tissue stained for PRPF3 using ab272596 at 1/200 in immunohistochemical analysis.
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PFA fixed, Triton X-100 permeablized human cell line A431 labeling PRPF3 (green) using ab272596 at 2 ug/ml in ICC/IF analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272596 は論文での使用が確認できていません。