Anti-PRPF3 抗体 (ab272596)
Key features and details
- Rabbit polyclonal to PRPF3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PRPF3 antibody -
製品の詳細
Rabbit polyclonal to PRPF3 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
-
免疫原
Recombinant fragment corresponding to Human PRPF3 aa 350-500.
Database link: O43395 -
ポジティブ･コントロール
- WB: HEK-293T cells. IHC-P: Human stomach tissue. ICC/IF: human cell line A431.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Cow
Orangutan
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. -
組織特異性
Highly expressed in retina, liver, kidney and blood. Detected at lower levels in heart and brain. -
関連疾患
Retinitis pigmentosa 18 -
配列類似性
Contains 1 PWI domain. -
細胞内局在
Nucleus speckle. Colocalizes with spliceosomal snRNPs. - Information by UniProt
-
参照データベース
- Entrez Gene: 510322 Cow
- Entrez Gene: 9129 Human
- Entrez Gene: 70767 Mouse
- Entrez Gene: 100440626 Orangutan
- Omim: 607301 Human
- SwissProt: Q2KIA6 Cow
- SwissProt: O43395 Human
- SwissProt: Q922U1 Mouse
see all -
別名
- hPrp3 antibody
- HPRP3P antibody
- Pre mRNA splicing factor 3 antibody
see all
画像
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Western blot - Anti-PRPF3 antibody (ab272596)All lanes : Anti-PRPF3 antibody (ab272596) at 0.4 µg/ml
Lane 1 : Control (vector only transfected HEK-293T lysate)
Lane 2 : PRPF3 over-expression lysate in mammalian HEK-293T cells -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF3 antibody (ab272596)Paraffin-embedded formalin-fixed Human stomach tissue stained for PRPF3 using ab272596 at 1/200 in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-PRPF3 antibody (ab272596)PFA fixed, Triton X-100 permeablized human cell line A431 labeling PRPF3 (green) using ab272596 at 2 ug/ml in ICC/IF analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272596 は論文での使用が確認できていません。
