Anti-PRP19 抗体 [EPR7446] (ab126776)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7446] to PRP19
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PRP19 antibody [EPR7446]
PRP19 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7446] to PRP19 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human PRP19 aa 450-550. The exact sequence is proprietary.
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ポジティブ・コントロール
- Human breast carcinoma and colon carcinoma tissues; HepG2, A431, MCF7, PC12, NIH 3T3, HeLa, K562, and U937 cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
解離定数(KD 値)
KD = 1.06 x 10 -10 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7446 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab126776の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Plays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells. -
組織特異性
Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines. -
配列類似性
Belongs to the WD repeat PRP19 family.
Contains 1 U-box domain.
Contains 7 WD repeats. -
細胞内局在
Nucleus. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin. - Information by UniProt
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参照データベース
- Entrez Gene: 27339 Human
- Entrez Gene: 28000 Mouse
- Entrez Gene: 246216 Rat
- Omim: 608330 Human
- SwissProt: Q9UMS4 Human
- SwissProt: Q99KP6 Mouse
- SwissProt: Q9JMJ4 Rat
- Unigene: 502705 Human
see all -
別名
- hPso4 antibody
- NMP200 antibody
- Nuclear matrix protein 200 antibody
see all
画像
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All lanes : Anti-PRP19 antibody [EPR7446] (ab126776) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : PC-12 cell lysate
Lane 5 : NIH/3T3 cell lysate
Lane 6 : HeLa cell lysate
Lane 7 : K562 cell lysate
Lane 8 : U937 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 55 kDa -
ab126776, at 1/50 dilution, staining PRP19 in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab126776, at 1/50 dilution, staining PRP19 in Paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab126776 は 2 報の論文で使用されています。
- Cai Y et al. Prp19 Is an Independent Prognostic Marker and Promotes Neuroblastoma Metastasis by Regulating the Hippo-YAP Signaling Pathway. Front Oncol 10:575366 (2020). PubMed: 33224878
- Guo J et al. Unraveling molecular effects of ADAR1 overexpression in HEK293T cells by label-free quantitative proteomics. Cell Cycle 15:1591-601 (2016). PubMed: 27104882