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RNA isolation procedure for cells
The only difference from the procedure in cells is the first step. Add 1 ml TRIzol to a sterile culture tube (preferably 12x75 mm). To this tube, add the frozen tissue (try not to add more than approximately 20 mg). On ice, pulverize the tissue with a homogenizer at a setting of 25 out of 30 for a total of 2 X 10 sec. Then pour the TRIzol solution into a 1.5 ml Eppendorf tube and proceed as above (from Step 4).
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