アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。
Take a look at our BETA site and see what we’ve done so far.
Search and browse selected products
Purchase these through your usual distributor
Dot blot is a technique for detecting, analyzing, and identifying proteins, similar to the western blot technique, but differing in that protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane or paper substrate.
The concentration of proteins in crude preparations (such as culture supernatant) can be estimated semiquantitatively using the dot blot method if you have both purified protein and specific antibody against it.
TBS:
20 mm Tris-HCl
150 mm NaCl
pH 7.5
TBS-T:
0.05% Tween 20 in TBS
BSA/TBS-T:
0.1% BSA in TBS-T
Nitrocellulose membrane (BIO-RAD, Trans-Blot, etc.)