製品の概要

  • 製品名
    Protein Quantitation Kit (Bradford Assay)
  • サンプルの種類
    Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts
  • アッセイタイプ
    Quantitative
  • 全工程の試験時間
    0h 20m
  • 製品の概要

    Abcam's Protein Quantitation Kit (Bradford Assay) provides a simple and rapid procedure for determining the concentration of protein in solution. The method utilizes an improved Coomassie blue G reagent which forms a blue complex in the presence of protein. The intensity of the blue complex is proportional to the amount of protein in the sample and can be easily measured by spectrophotometer or plate reader at 595 nm.

法規制情報

製品の特性

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  • Colorimetric standard curves of 150 microliter samples: mean of duplicates with background readings subtracted.

  • Colorimetric standard curves of 100 microliter samples: mean of duplicates with background readings subtracted

プロトコール

参考文献

This product has been referenced in:
  • Jabarpour M  et al. Hyperbilirubinemia-induced pro-angiogenic activity of infantile endothelial progenitor cells. Microvasc Res 118:49-56 (2018). Read more (PubMed: 29476756) »
  • Shi Q  et al. KLF5 promotes apoptosis induced by phorbol ester as an effector of the autocrine factor TNFa in LNCaP prostate cancer cells. Oncol Lett 14:1847-1854 (2017). Read more (PubMed: 28789420) »

See all 4 Publications for this product

レビューと Q&A

I can confirm that both BCA and Bradford protein quantification kits can be used for human serum and plasma samples. The main difference between the two kits is that the BCA assay uses a Copper ion based chemistry whereas the Bradford assay uses a Coom...

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Thank you for contacting us and your interest in our products.

The two kits you have chosen should be suitable alternatives for the customer wanting to quantify protein in their samples.

You may also want to bring the BCA Protein Quan...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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