Bradford Assay Kit (ab102535)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Cell culture extracts, Other biological fluids, Plasma, Serum, Tissue Extracts
製品の概要
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製品名
Bradford Assay Kit -
検出方法
Colorimetric -
サンプルの種類
Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts -
アッセイタイプ
Quantitative -
全工程の試験時間
0h 20m -
製品の概要
Bradford Assay Kit ab102535 provides a simple and rapid procedure for determining the concentration of protein in solution.
The Bradford assay protocol uses an improved Coomassie blue G reagent which forms a blue complex in the presence of protein. The intensity of the blue complex is proportional to the amount of protein in the sample and can be easily measured by spectrophotometer or plate reader at 595 nm.
Bradford assay protocol summary:
- prepare serial dilutions of protein standard
- add samples and standards to wells or tubes
- add Bradford protein assay solution and incubate for 5 min at room temp
- measure absorbance at 595 nm
Support on how to determine protein concentration can be found here
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K810 Protein Quantitation Kit. K810-1000 is the same size as the 1000 test size of ab102535.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1000 tests 5X Protein Assay Solution 1 x 20ml Protein Standard I 1 x 1.5ml -
研究分野
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関連性
Protein Quantitation is a procedure for determining the concentration of protein in solution.
画像
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Colorimetric standard curves of 150 microliter samples: mean of duplicates with background readings subtracted.
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Colorimetric standard curves of 100 microliter samples: mean of duplicates with background readings subtracted.
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Colorimetric standard curve of 10 microliter samples: mean of duplicates with background readings subtracted.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (25)
ab102535 は 25 報の論文で使用されています。
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- Luo J et al. miR-642a-5p increases glucocorticoid sensitivity by suppressing the TLR4 signalling pathway in THP-1 cells. Biochem Biophys Rep 32:101356 (2022). PubMed: 36186733
- Suleimenov M et al. Bcl-xL activity influences outcome of the mitotic arrest. Front Pharmacol 13:933112 (2022). PubMed: 36188556