Protein G Sepharose® (ab193259)
Key features and details
- Sample type: Ascites Fluid, Cell culture media, Cell culture supernatant, Serum
製品の概要
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製品名
Protein G Sepharose® -
サンプルの種類
Cell culture supernatant, Serum, Cell culture media, Ascites Fluid -
製品の概要
Contents:
Supplied as 50% slurry in 20% Ethanol.
Features:
High binding capacity: Binding of IgG >20 mg human or rabbit IgG/mL Protein G Sepharose®.
Minimal Leaching of the Ligand
Flow Rate Tested*: 2.07 mL/min.
*Test condition: = Calculations based on the time required to pass 18 mL of water through 2 mL settled beads (column diameter 1.5 cm).
Usage: Reusable for up to 10 times without significant loss of binding capacity.
These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
Sepharose is a registered trademark of GE Healthcare
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 6511 Protein G Sepharose. 6511-100 is the same size as the 100 ml size of ab193259.
Protein G Sepharose® beads are prepared by covalently coupling recombinant Protein G to 6% cross-linked Sepharose® beads. Protein G is a genetically engineered protein containing three IgG-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG and minimum leaching of recombinant Protein G. The IgG binding capacity of Protein G Sepharose® is >20 mg of human or rabbit IgG per mL of wet beads. Protein G Sepharose® beads display high chemical and physical stability as well as high flow rate, hydrophilicity and high gel strength. This product can be used for IgG purification and immunoprecipitation.
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アプリケーション
適用あり: Purification, IPmore details
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 ml 5 ml 25 ml 100 ml Protein G Sepharose® 1 x 1ml 1 x 5ml 1 x 25ml 1 x 100ml -
研究分野
関連製品
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab193259の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Purification |
Use at an assay dependent concentration.
Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. |
|
| IP |
Use at an assay dependent concentration.
Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen. |
| 特記事項 |
|---|
|
Purification
Use at an assay dependent concentration. Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. |
|
IP
Use at an assay dependent concentration. Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen. |
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab193259 は 6 報の論文で使用されています。
- Filippou A et al. ANO1 Expression Orchestrates p27Kip1/MCL1-Mediated Signaling in Head and Neck Squamous Cell Carcinoma. Cancers (Basel) 13:N/A (2021). PubMed: 33803266
- Miccoli A et al. Molecular, Cellular and Functional Analysis of TR? Chain along the European Sea Bass Dicentrarchus labrax Development. Int J Mol Sci 22:N/A (2021). PubMed: 33806063
- Sergeeva O et al. Modification of Adenosine196 by Mettl3 Methyltransferase in the 5'-External Transcribed Spacer of 47S Pre-rRNA Affects rRNA Maturation. Cells 9:N/A (2020). PubMed: 32344536
- Fefilova A et al. Murine Long Noncoding RNA Morrbid Contributes in the Regulation of NRAS Splicing in Hepatocytes In Vitro. Int J Mol Sci 21:N/A (2020). PubMed: 32764370
- Mohan HM et al. Calreticulin enhances the secretory trafficking of a misfolded a-1-antitrypsin. J Biol Chem 295:16754-16772 (2020). PubMed: 32978262
- DeLeo FR et al. Survival of Carbapenem-Resistant Klebsiella pneumoniae Sequence Type 258 in Human Blood. Antimicrob Agents Chemother 61:N/A (2017). Human . PubMed: 28115349