Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5835] to Proteasome subunit alpha type 6 - BSA and Azide free
- Suitable for: ICC, Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Rat, Human
製品名Anti-Proteasome subunit alpha type 6 antibody [EPR5835] - BSA and Azide free
Proteasome subunit alpha type 6 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5835] to Proteasome subunit alpha type 6 - BSA and Azide free
アプリケーション適用あり: ICC, Flow Cyt, IHC-P, WBmore details
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human Proteasome subunit alpha type 6 aa 200-300 (C terminal). The exact sequence is proprietary.
Database link: P60900
Ab247839 is the carrier-free version of ab109377. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247839 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab247839 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.
|WB||Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).|
機能The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
配列類似性Belongs to the peptidase T1A family.
- Information by UniProt
- 27 kDa prosomal protein antibody
- IOTA antibody
- Macropain iota chain antibody
This data was developed using ab109377, the same antibody clone in a different buffer formulation.
ab109377, at a 1/250 dilution, staining Proteasome 20S alpha 6 in paraffin embedded Human colon tissue by Immunohistochemistry.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.