Proteasome Activity Assay Kit (ab107921)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell Lysate
製品の概要
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製品名
Proteasome Activity Assay Kit -
検出方法
Fluorescent -
サンプルの種類
Cell Lysate -
アッセイタイプ
Enzyme activity -
全工程の試験時間
1h 00m -
種交差性
交差種: Mammals, Other species -
製品の概要
Proteasome Activity Assay Kit (ab107921) takes advantage of the chymotrypsin-like activity, using an AMC-tagged peptide substrate (Proteasome Substrate (Succ-LLVY-AMC in DMSO), which releases free, highly fluorescent AMC (Ex/Em 350/440 nm) in the presence of proteolytic activity.
The kit also includes a positive control (Jurkat Cell lysate with significant proteasome activity) and a specific proteasome inhibitor MG-132 which suppresses all proteolytic activity due to proteasomes. This permits differentiation of proteasome activity from other protease activity which may be present in samples.
Proteasome activity assay protocol summary:
- add samples, standards and positive control to wells
- add proteasome inhibitor to half of sample and positive control wells
- add proteasome substrate to sample and control wells
- incubate for 20 min
- analyze with microplate reader, incubate for further 30 min, analyze again -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K245 Proteasome Activity Fluorometric Assay Kit. K245-100 is the same size as the 100 test size of ab107921.
The 20S proteasome assembly is the functional protease structure with chymotrypsin-like, trypsin-like and caspase-like protease activities
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests AMC Standard 1 x 100µl Proteasome Assay Buffer 1 x 25ml Proteasome Inhibitor 1 x 100µl Proteasome Positive Control 1 vial Proteasome Substrate 1 x 100µl -
研究分野
画像
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Proteasome Activity measured in Jurkat lysate
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Proteasome Activity measured in HeLa lysate
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Proteasome Activity measured in cell lysates showing activity (mU) per 1 mln cells.
Samples with the concentration of 3.3e6 cells/mL were used. 50 μl of each sample was used.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (69)
ab107921 は 69 報の論文で使用されています。
- Wang W et al. Analysis of a degron-containing reporter protein GFP-CL1 reveals a role for SUMO1 in cytosolic protein quality control. J Biol Chem 299:102851 (2023). PubMed: 36587767
- Cheng KC et al. Early Aß42 Exposure Causes Learning Impairment in Later Life. Aging Dis 13:868-883 (2022). PubMed: 35656119
- Chen F et al. Comparative maternal protein profiling of mouse biparental and uniparental embryos. Gigascience 11:N/A (2022). PubMed: 36056732
- Nagendra AH et al. Sodium fluoride induces skeletal muscle atrophy via changes in mitochondrial and sarcomeric proteomes. PLoS One 17:e0279261 (2022). PubMed: 36548359
- Deng X et al. CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1. J Cell Biol 221:N/A (2022). PubMed: 34726688