Key features and details
- Rabbit polyclonal to Proteasome Activator Subunit 4/PSME4
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Cow, Human
- Isotype: IgG
製品名Anti-Proteasome Activator Subunit 4/PSME4 antibody
Proteasome Activator Subunit 4/PSME4 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Proteasome Activator Subunit 4/PSME4
アプリケーション適用あり: ICC/IF, WBmore details
種交差性交差種: Mouse, Cow, Human
This product was previously labelled as Proteasome Activator Subunit 4
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.05% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab5620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 200 kDa.|
機能Activates proteasomal cleavage of peptides in an energy-independent manner. May be involved in spermatogenesis. May be involved in DNA repair.
配列類似性Contains 6 HEAT repeats.
細胞内局在Nucleus. Nucleus speckle. Found in nuclear foci following treatment with ionizing radiation, but not with ultraviolet irradiation or H(2).
- Information by UniProt
- KIAA0077 antibody
- PA200 antibody
- Proteasome Activator 200 kDa antibody
Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620) at 1/2000 dilution + Mouse testis tissue lysate at 25 µg
Developed using the ECL technique.
Observed band size: 160,200 kDa why is the actual band size different from the predicted?
ICC/IF image of ab5620 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab5620 は 3 報の論文で使用されています。
- Pickering AM & Davies KJ Differential roles of proteasome and immunoproteasome regulators Pa28aß, Pa28? and Pa200 in the degradation of oxidized proteins. Arch Biochem Biophys 523:181-90 (2012). WB ; Mouse . PubMed: 22564544
- Greenhaff PL et al. Disassociation between the effects of amino acids and insulin on signaling, ubiquitin ligases, and protein turnover in human muscle. Am J Physiol Endocrinol Metab 295:E595-604 (2008). WB ; Human . PubMed: 18577697
- Zhang L et al. Effects of aging and dietary restriction on ubiquitination, sumoylation, and the proteasome in the spleen. FEBS Lett 581:5543-7 (2007). PubMed: 17991438