Key features and details
- Rabbit polyclonal to Proteasome 20S LMP2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品名Anti-Proteasome 20S LMP2 antibody
Proteasome 20S LMP2 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Proteasome 20S LMP2
特異性Detects proteasome 20S LMP2.
アプリケーション適用あり: WBmore details
種交差性交差種: Mouse, Human
交差が予測される動物種: Rat, Cow
- WB: HeLa cells, mouse embryo fibroblast cells
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーConstituents: 0.1% BSA, 99% PBS
Concentration information loading...
精製度Immunogen affinity purified
機能The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.
配列類似性Belongs to the peptidase T1B family.
発生段階Highly expressed in immature dendritic cells (at protein level).
翻訳後修飾Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.
- Information by UniProt
- Beta1i antibody
- Large multifunctional peptidase 2 antibody
- Large multifunctional protease 2 antibody
All lanes : Anti-Proteasome 20S LMP2 antibody (ab3328) at 2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/mL IFN gamma for 72 hours, whole cell lysate
Lane 3 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 4 : THP-1 (human monocytic leukemia cell line) treated with 50 ng/mL PMA for 48 hours and 200 ng/mL LPS for 24 hours, whole cell lysate
Lane 5 : U937 (human histiocytic lymphoma cell line) whole cell lysate
Lane 6 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 7 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 0.4 µg/ml
Developed using the ECL technique.
Western blot of proteasome 20S LMP2 in 50 ug mouse embryo fibroblast cell extract using ab3328.
ab3328 は 28 報の論文で使用されています。
- Kalaora S et al. Immunoproteasome expression is associated with better prognosis and response to checkpoint therapies in melanoma. Nat Commun 11:896 (2020). PubMed: 32060274
- Lee MJ et al. H727 cells are inherently resistant to the proteasome inhibitor carfilzomib, yet require proteasome activity for cell survival and growth. Sci Rep 9:4089 (2019). PubMed: 30858500
- Jimenez-Guardeño JM et al. Immunoproteasome activation enables human TRIM5a restriction of HIV-1. Nat Microbiol 4:933-940 (2019). PubMed: 30886358
- Babaer D et al. Methylselenol producing selenocompounds enhance the efficiency of mammaglobin-A peptide vaccination against breast cancer cells. Oncol Lett 18:6891-6898 (2019). PubMed: 31807192
- Studencka-Turski M et al. Molecular Insight Into the IRE1a-Mediated Type I Interferon Response Induced by Proteasome Impairment in Myeloid Cells of the Brain. Front Immunol 10:2900 (2019). PubMed: 31921161