Anti-Proteasome 20S C2/HC2 抗体 [EPR5451] - BSA and Azide free (ab247881)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5451] to Proteasome 20S C2/HC2 - BSA and Azide free
- Suitable for: ICC, IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
製品の概要
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製品名
Anti-Proteasome 20S C2/HC2 antibody [EPR5451] - BSA and Azide free
Proteasome 20S C2/HC2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5451] to Proteasome 20S C2/HC2 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: ICC, IP, IHC-P, WBmore details
適用なし: Flow Cyt -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Proteasome 20S C2/HC2 aa 1-100. The exact sequence is proprietary.
Database link: P25786 -
特記事項
ab247881 is the carrier-free version of ab109500 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247881 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Proteasome 20S C2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5451 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab247881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval.
|
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| WB | Use at an assay dependent concentration. Predicted molecular weight: 30 kDa. |
ターゲット情報
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機能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells. -
配列類似性
Belongs to the peptidase T1A family. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5682 Human
- Entrez Gene: 26440 Mouse
- Entrez Gene: 29668 Rat
- Omim: 602854 Human
- SwissProt: P25786 Human
- SwissProt: Q9R1P4 Mouse
- SwissProt: P18420 Rat
- Unigene: 102798 Human
see all -
別名
- 30 kDa prosomal protein antibody
- HC 2 antibody
- HC2 antibody
see all
画像
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![Western blot - Anti-Proteasome 20S C2/HC2 antibody [EPR5451] - BSA and Azide free (ab247881)](https://www.abcam.co.jp/ps/products/247/ab247881/Images/ab247881-398914-anti-proteasome-20s-c2-hc2-antibody-epr5451-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Proteasome 20S C2/HC2 antibody [EPR5451] - BSA and Azide free (ab247881)All lanes : Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : PC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDaThis data was developed using ab109500, the same antibody clone in a different buffer formulation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
Certificate of Compliance
参考文献
ab247881 は論文での使用が確認できていません。
