Anti-Proteasome 20S C2/HC2 抗体 [EPR5451] (ab109500)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500)](https://www.abcam.co.jp/ps/products/109/ab109500/Images/Proteasome-20S-C2-Primary-antibodies-ab109500-4.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5451] to Proteasome 20S C2/HC2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Proteasome 20S C2/HC2 antibody [EPR5451]
Proteasome 20S C2/HC2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5451] to Proteasome 20S C2/HC2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HeLa, Jurkat, HepG2, and PC-3 cell lysates Human breast tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5451 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109500の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval |
| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 30 kDa. |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval |
追加情報
Is unsuitable for Flow Cyt.
ターゲット情報
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機能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells. -
配列類似性
Belongs to the peptidase T1A family. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5682 Human
- Entrez Gene: 26440 Mouse
- Entrez Gene: 29668 Rat
- Omim: 602854 Human
- SwissProt: P25786 Human
- SwissProt: Q9R1P4 Mouse
- SwissProt: P18420 Rat
- Unigene: 102798 Human
see all -
別名
- 30 kDa prosomal protein antibody
- HC 2 antibody
- HC2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500)
Immunohistochemical analysis of paraffin-embedded Human breast tissue using ab109500 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500)](https://www.abcam.co.jp/ps/products/109/ab109500/Images/Proteasome-20S-C2-Primary-antibodies-ab109500-1.jpg)
Western blot - Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500)All lanes : Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : PC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa -
![Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500)](https://www.abcam.co.jp/ps/products/109/ab109500/Images/ab109500-3-benefits-of-recombinant-antibodies.png)
Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500)
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab109500 は 1 報の論文で使用されています。
- Yang Q et al. Proteomic profiling of antibody-inducing immunogens in tumor tissue identifies PSMA1, LAP3, ANXA3, and maspin as colon cancer markers. Oncotarget 9:3996-4019 (2018). PubMed: 29423100
