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    proteasome-20s-c2hc2-antibody-ab3325.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome
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Anti-Proteasome 20S C2/HC2 抗体 (ab3325)

  • Datasheet
Reviews (2)Q&A (1)References (28)

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Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
  • Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
  • Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab3325)

Key features and details

  • Rabbit polyclonal to Proteasome 20S C2/HC2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Hamster, Dog, Human, Chinese hamster
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Proteasome 20S C2/HC2 antibody [EPR5451] (ab109500)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Proteasome 20S C2/HC2 antibody
    Proteasome 20S C2/HC2 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Proteasome 20S C2/HC2
  • 由来種

    Rabbit
  • 特異性

    Detects proteasome 20S C2/HC2 subunit.

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Rat, Hamster, Dog, Human, Chinese hamster
    交差が予測される動物種: Chicken, Cow, Cynomolgus monkey
  • 免疫原

    Synthetic peptide corresponding to Human Proteasome 20S C2/HC2 aa 249-263 (C terminal).
    Sequence:

    PADEPAEKADEPMEH


    Database link: P25786
    (Peptide available as ab4943)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ・コントロール

    • WB: MDA-MB-231, MCF7, PC-3, HepG2 and Jurkat whole cell lysate, CHO whole cell lysate. ICC/IF: MDA-MB-231 cells.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome
    • Neuroscience
    • Processes

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Human Proteasome 20S C2/HC2 protein (ab116156)
  • Recombinant Protein

    • Recombinant Human Proteasome 20S C2/HC2 protein (ab116156)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3325の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use a concentration of 1 - 3 µg/ml.
IHC-P
Use at an assay dependent concentration.
ICC/IF (1)
Use a concentration of 2 µg/ml.
特記事項
WB
Use a concentration of 1 - 3 µg/ml.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 2 µg/ml.

ターゲット情報

  • 機能

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells.
  • 配列類似性

    Belongs to the peptidase T1A family.
  • 細胞内局在

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession P25786 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 395874 Chicken
    • Entrez Gene: 515503 Cow
    • Entrez Gene: 476867 Dog
    • Entrez Gene: 5682 Human
    • Entrez Gene: 26440 Mouse
    • Entrez Gene: 29668 Rat
    • Omim: 602854 Human
    • SwissProt: O42265 Chicken
    • SwissProt: Q3T0X5 Cow
    • SwissProt: P25786 Human
    • SwissProt: Q9R1P4 Mouse
    • SwissProt: P18420 Rat
    • Unigene: 102798 Human
    • Unigene: 121265 Mouse
    • Unigene: 2668 Rat
    see all
  • 別名

    • 30 kDa prosomal protein antibody
    • HC 2 antibody
    • HC2 antibody
    • Macropain subunit C2 antibody
    • Macropain subunit nu antibody
    • MGC14542 antibody
    • MGC14575 antibody
    • MGC14751 antibody
    • MGC1667 antibody
    • MGC21459 antibody
    • MGC22853 antibody
    • MGC23915 antibody
    • Multicatalytic endopeptidase complex subunit C2 antibody
    • NU antibody
    • PROS 30 antibody
    • PROS-30 antibody
    • PROS30 antibody
    • Proteasome (prosome macropain) subunit alpha type 1 antibody
    • Proteasome alpha 1 subunit antibody
    • Proteasome component C2 antibody
    • Proteasome nu chain antibody
    • Proteasome subunit alpha type 1 antibody
    • Proteasome subunit alpha type I antibody
    • Proteasome subunit alpha type-1 antibody
    • Proteasome subunit nu antibody
    • Protein P30 33K antibody
    • PSA1_HUMAN antibody
    • PSC 2 antibody
    • PSC2 antibody
    • PSMA 1 antibody
    • psmA1 antibody
    see all

画像

  • Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
    Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
    All lanes : Anti-Proteasome 20S C2/HC2 antibody (ab3325) at 2 µg/ml

    Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) HRP cpnjugate at 0.4 µg/ml

    Developed using the ECL technique.


    Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.

  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
    Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S C2/HC2 antibody (ab3325)

    Immunofluorescence analysis of 70% confluent log phase MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Proteasome 20S C2/HC2 (green) with ab3325 at 2 µg/mL. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab3325 in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the control without primary antibody. The images were captured at 60X magnification.

  • Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
    Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
    All lanes : Anti-Proteasome 20S C2/HC2 antibody (ab3325) at 2 µg/ml

    Lane 1 : Untransfected Hep G2 whole cell extract.
    Lane 2 : Proteasome 20S C2/HC2 non-targeting scrambled siRNA transfected Hep G2 whole cell extract.
    Lane 3 : Proteasome 20S C2/HC2 knockdown Hep G2 whole cell extract.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
    Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab3325)
    Anti-Proteasome 20S C2/HC2 antibody (ab3325) at 3 µg/ml + CHO (Chinese hamster ovary cell line) whole cell lysate

プロトコール

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

  • Datasheet download

    Download

参考文献 (28)

ab3325 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3325 は 28 報の論文で使用されています。

  • Christensen IB  et al. Genetic disruption of slc4a10 alters the capacity for cellular metabolism and vectorial ion transport in the choroid plexus epithelium. Fluids Barriers CNS 17:2 (2020). PubMed: 31906971
  • Murali SK  et al. The Deubiquitylase USP4 Interacts with the Water Channel AQP2 to Modulate Its Apical Membrane Accumulation and Cellular Abundance. Cells 8:N/A (2019). PubMed: 30901874
  • Saglar Ozer E  et al. Molecular characterization of an aquaporin-2 mutation causing a severe form of nephrogenic diabetes insipidus. Cell Mol Life Sci N/A:N/A (2019). PubMed: 31302751
  • Sun C  et al. Cellular Requirements for Sensing and Elimination of Incoming HSV-1 DNA and Capsids. J Interferon Cytokine Res 39:191-204 (2019). PubMed: 30855198
  • Auvin S  et al. A molecule inducing androgen receptor degradation and selectively targeting prostate cancer cells. Life Sci Alliance 2:N/A (2019). PubMed: 31431473
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Proteasome 20S C2/HC2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (RPE-1)
Permeabilization
Yes - 0.5% Triton X100 in PBS
Specification
RPE-1
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

投稿 Oct 26 2007

Western blot abreview for Anti-Proteasome 20S C2/HC2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (heart cells)
Specification
heart cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. ping wang

Verified customer

投稿 Jan 27 2006

Question

Is Proteasome 20s C2 the same protein as Proteasome subunit alpha type I, with swissprot nº P25786?

Read More

Abcam community

Verified customer

Asked on Oct 13 2003

Answer

Yes, Proteasome 20s C2 is the same as Proteasome subunit alpha type I. I just BLASTed the immunogen sequence and the sequence is pulls out is the SwissProt accession number that you mentioned.

Read More

Asdf Edo

Abcam Scientific Support

Answered on Oct 13 2003

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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