Anti-Proteasome 20S beta 6 抗体 (ab3331)
Key features and details
- Rabbit polyclonal to Proteasome 20S beta 6
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Cow, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Proteasome 20S beta 6 antibody
Proteasome 20S beta 6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Proteasome 20S beta 6 -
由来種
Rabbit -
特異性
Detects proteasome 20S beta 6 from purified bovine and human 26S proteasome samples. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Cow, Human
交差が予測される動物種: Rat, Xenopus laevis, Zebrafish -
免疫原
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ポジティブ・コントロール
- WB: HeLa, BAEC whole cell lysates. ICC: A431, HeLa, NIH 3T3, BAEC cells.
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特記事項
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 3% Sodium deoxycholate, 3% Triton-X-100, 0.3% Tris HCl, 15% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab3331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | 1/10 - 1/100. | |
WB | Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
ターゲット情報
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機能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the peptidyl glutamyl-like activity. May catalyze basal processing of intracellular antigens. -
配列類似性
Belongs to the peptidase T1B family. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5694 Human
- Entrez Gene: 19175 Mouse
- Entrez Gene: 100360846 Rat
- Entrez Gene: 100364558 Rat
- Entrez Gene: 29666 Rat
- Entrez Gene: 30388 Zebrafish
- Omim: 600307 Human
- SwissProt: P28072 Human
see all -
別名
- DELTA antibody
- LMPY antibody
- Macropain delta chain antibody
see all
画像
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All lanes : Anti-Proteasome 20S beta 6 antibody (ab3331) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial adenocarcinoma cell line) whole cell lysate
Lane 2 : BAEC (Bovine aortic endothelial cell line) whole cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated secondary antibody
Predicted band size: 25 kDa -
Immunocytochemistry/Immunofluorescence analysis of Proteasome 20S beta 6 (green) showing staining in the cytoplasm and nucleus of A431 (Human epidermoid carcinoma cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3331 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunofluorescent analysis of Proteasome 20S Y (green) in NIH/3T3 (Mouse embryo fibroblast cell line) cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes and blocked with 3% Blocker BSA in PBS for 30 minutes at room temperature. Cells were stained with or without Proteasome 20S Y rabbit polyclonal antibody, at a concentration of 5 µg/mL for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at 1/1000 dilution for 1 hour at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
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Immunocytochemistry/Immunofluorescence analysis of Proteasome 20S beta 6 (green) showing staining in the cytoplasm and nucleus of BAEC (Bovine aortic endothelial cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3331 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/Immunofluorescence analysis of Proteasome 20S beta 6 (green) showing staining in the cytoplasm and nucleus of HeLa (Human epithelial adenocarcinoma cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3331 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
プロトコール
参考文献 (1)
ab3331 は 1 報の論文で使用されています。
- de Verteuil D et al. Deletion of immunoproteasome subunits imprints on the transcriptome and has a broad impact on peptides presented by major histocompatibility complex I molecules. Mol Cell Proteomics 9:2034-47 (2010). WB ; Mouse . PubMed: 20484733