Anti-Proteasome 20S alpha 2/HC3 抗体 [MCP21] (ab22666)
![Electrophoretic Mobility Shift Assay - Anti-Proteasome 20S alpha 2/HC3 antibody [MCP21] (ab22666)](https://www.abcam.co.jp/ps/products/22/ab22666/Images/ab22666-32457-anti-proteasome-20s-alpha-2-antibody-mcp21-electrophoretic-mobility-shift-assay.jpg "Electrophoretic Mobility Shift Assay - Anti-Proteasome 20S alpha 2/HC3 antibody [MCP21] (ab22666)")
Key features and details
- Mouse monoclonal [MCP21] to Proteasome 20S alpha 2/HC3
- Suitable for: WB, Flow Cyt, EMSA
- Reacts with: Rabbit, Human
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Proteasome 20S alpha 2/HC3 antibody [MCP21]
Proteasome 20S alpha 2/HC3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MCP21] to Proteasome 20S alpha 2/HC3 -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cyt, EMSAmore details -
種交差性
交差種: Rabbit, Human
非交差種: Mouse, Rat -
免疫原
Full length protein corresponding to Human Proteasome 20S alpha 2/HC3. Human placenta derived proteasomes
Database link: P25787 -
ポジティブ･コントロール
- human placental proteasome preparation and HeLa cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.065% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
IgG fraction -
ポリ/モノ
モノクローナル -
クローン名
MCP21 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab22666の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration.
|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
|
| EMSA |
1/10000.
|
| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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|
EMSA
1/10000. |
ターゲット情報
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機能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation. -
配列類似性
Belongs to the peptidase T1A family. -
翻訳後修飾
Phosphorylated on tyrosine residues; which may be important for nuclear import. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5683 Human
- Omim: 176842 Human
- SwissProt: P25787 Human
- Unigene: 333786 Human
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別名
- HC3 antibody
- Macropain subunit C3 antibody
- MU antibody
see all
画像
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Electrophoretic Mobility Shift Assay - Anti-Proteasome 20S alpha 2/HC3 antibody [MCP21] (ab22666)Luminograph of human placental proteasome preparation after 2D PADE followed by blotting onto nitrocellulose and probing with ab22666 (1/1000). ECL exposure , 1 minute exposure).
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![Flow Cytometry - Anti-Proteasome 20S alpha 2/HC3 antibody [MCP21] (ab22666)](https://www.abcam.co.jp/ps/products/22/ab22666/Images/ab22666-32455-anti-proteasome-20s-alpha-2-antibody-mcp21-flow-cytometry.jpg)
Flow Cytometry - Anti-Proteasome 20S alpha 2/HC3 antibody [MCP21] (ab22666)Overlay histogram showing HeLa cells stained with ab22666 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22666, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab22666 は 2 報の論文で使用されています。
- Wiemhoefer A et al. Tripeptidyl Peptidase II Mediates Levels of Nuclear Phosphorylated ERK1 and ERK2. Mol Cell Proteomics 14:2177-93 (2015). PubMed: 26041847
- Juenemann K et al. Expanded polyglutamine-containing N-terminal huntingtin fragments are entirely degraded by mammalian proteasomes. J Biol Chem 288:27068-84 (2013). PubMed: 23908352
