Anti-Proteasome 20S alpha 2/HC3 抗体 [EPR5454] (ab109502)
![Western blot - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454] (ab109502)](https://www.abcam.co.jp/ps/products/109/ab109502/Images/Proteasome-20S-alpha-2-Primary-antibodies-ab109502-1.jpg "Western blot - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454] (ab109502)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5454] to Proteasome 20S alpha 2/HC3
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454]
Proteasome 20S alpha 2/HC3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5454] to Proteasome 20S alpha 2/HC3 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Jurkat, 293T, HeLa or JAR cell lysate. Flow Cyt (intra): HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5454 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109502の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Predicted molecular weight: 26 kDa. |
追加情報
Is unsuitable for ICC,IHC-P or IP.
ターゲット情報
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機能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation. -
配列類似性
Belongs to the peptidase T1A family. -
翻訳後修飾
Phosphorylated on tyrosine residues; which may be important for nuclear import. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5683 Human
- Entrez Gene: 19166 Mouse
- Entrez Gene: 29669 Rat
- Omim: 176842 Human
- SwissProt: P25787 Human
- SwissProt: P49722 Mouse
- SwissProt: P17220 Rat
- Unigene: 333786 Human
see all -
別名
- HC3 antibody
- Macropain subunit C3 antibody
- MU antibody
see all
画像
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Western blot - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454] (ab109502)All lanes : Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454] (ab109502) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : JAR cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDa -
![Flow Cytometry (Intracellular) - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454] (ab109502)](https://www.abcam.co.jp/ps/products/109/ab109502/Images/Proteasome-20S-alpha-2-Primary-antibodies-ab109502-3.jpg)
Flow Cytometry (Intracellular) - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454] (ab109502)Intracellular flow cytometric analysis of permeabilized HeLa cells using ab109502 at 1/10 dilution (red) and a control rabbit IgG (green).
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![Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454] (ab109502)](https://www.abcam.co.jp/ps/products/109/ab109502/Images/ab109502-3-benefits-of-recombinant-antibodies.png)
Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454] (ab109502)
データシートおよび資料
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Datasheet download
参考文献 (2)
ab109502 は 2 報の論文で使用されています。
- Zhao WT et al. The Yunnan national medicine Maytenus compound inhibits the proliferation of hepatocellular carcinoma (HCC) by suppressing the activation of the EGFR-PI3K-AKT signaling pathway. J Cancer 12:3325-3334 (2021). PubMed: 33976742
- Niu Y et al. Huaier Suppresses the Hepatocellular Carcinoma Cell Cycle by Regulating Minichromosome Maintenance Proteins. Onco Targets Ther 13:12015-12025 (2020). PubMed: 33244243
