Application
Western blot
Sample
Rat Tissue lysate - whole (Prefrontal cortex)
Loading amount
20 µg
Specification
Prefrontal cortex
Gel Running Conditions
Non-reduced Denaturing (10% acrylamide)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Other product details
Dilution
1/2000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 30 kDa
Positive control
my samples
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 11 2011