Anti-Proteasome 19S S4/PSMC1 抗体 (ab3317)

Reviews (1)Q&A (3)References (4)
Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (ab3317)

    Key features and details

    • Rabbit polyclonal to Proteasome 19S S4/PSMC1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-Proteasome 19S S4/PSMC1 antibody
      Proteasome 19S S4/PSMC1 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to Proteasome 19S S4/PSMC1

    • 由来種

      Rabbit

    • 特異性

      Detects proteasome 19S subunit S4.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Full length protein corresponding to Proteasome 19S S4/PSMC1. Yeast proteasome 19S subunit S4.

    • ポジティブ･コントロール

      • WB: HeLa cell lysate.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...

    • 精製度

      IgG fraction

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab3317の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 49 kDa).

    This antibody detects a 56 kDa protein representing proteasome 19S subunit S4 in HeLa cell lysate. This antibody detects, to a lesser extent, an ~35 kDa protein which could correspond to subunit degradation product.
    特記事項
    WB
    1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 49 kDa).

    This antibody detects a 56 kDa protein representing proteasome 19S subunit S4 in HeLa cell lysate. This antibody detects, to a lesser extent, an ~35 kDa protein which could correspond to subunit degradation product.

    ターゲット情報

    • 機能

      The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.

    • 配列類似性

      Belongs to the AAA ATPase family.

    • 細胞内局在

      Cytoplasm. Nucleus.
    • Information by UniProt

    • 参照データベース

      • 別名

        • 26S protease regulatory subunit 4 antibody
        • 26S proteasome AAA-ATPase subunit RPT2 antibody
        • MGC24583 antibody
        • MGC8541 antibody
        • P26s4 antibody
        • p56 antibody
        • protease 26S, subunit 4 antibody
        • Proteasome (prosome macropain) 26S subunit ATPase 1 antibody
        • Proteasome 26S ATPase subunit 1 antibody
        • Proteasome 26S subunit ATPase 1 antibody
        • PRS4_HUMAN antibody
        • PSMC 1 antibody
        • Psmc1 antibody
        • S4 antibody
        see all

      画像

      • Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (ab3317)
        Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (ab3317)

        Western blot of proteasome 19S subunit S4 on HeLa cell lysate using ab3317.

      参考文献 (4)

      ab3317 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab3317 は 4 報の論文で使用されています。

      • Adelöf J  et al. PA28aß overexpression enhances learning and memory of female mice without inducing 20S proteasome activity. BMC Neurosci 19:70 (2018). PubMed: 30400847
      • Zhou X  et al. HIV Replication in CD4+ T Lymphocytes in the Presence and Absence of Follicular Dendritic Cells: Inhibition of Replication Mediated by {alpha}-1-Antitrypsin through Altered I{kappa}B{alpha} Ubiquitination. J Immunol 186:3148-55 (2011). WB, IP . PubMed: 21263074
      • Rousseau E  et al. Misfolding of proteins with a polyglutamine expansion is facilitated by proteasomal chaperones. J Biol Chem 284:1917-29 (2009). WB, ICC/IF ; Human, Rat . PubMed: 18986984
      • Sun X  et al. Molecular characterization of tumour heterogeneity and malignant mesothelioma cell differentiation by gene profiling. J Pathol 207:91-101 (2005). PubMed: 16007577

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      1-4 of 4 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Proteasome 19S S4 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Blocking step
      BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
      Sample
      Aedes aegypti (Mosquito) Cell (c6/36)
      Specification
      c6/36
      Permeabilization
      Yes - .01 % TritonX100
      Fixative
      Paraformaldehyde
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      Abcam user community

      Verified customer

      投稿 Nov 25 2014

      Question

      I am using this antibody in WB. I have tested a broad range of protein concentrations (1 - 100ug K562 cells) and dilutions (1:1000 - 1:10000). I would like to receive a replacement antibody.

      Read More
      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

      To check the status of the order please contact our Customer Service team and reference this number.

      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

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      Question

      I have a question about Proteasome 19S S4 antibody (ab3317). The data sheet says the immunogen used was full length protein from S. cerevisiae. Can you tell me if this was a tagged protein, and if so was it tagged at the N or C-termnus. I ask because this is an N-myristoylated protein (myristoylated on the N-terminal Gly) and I want to know if the immunogen from yeast (which normally has myristoylation activity) would then be the myristoylated form used to make your antibody. Thanks

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      Answer

      Thank you for your enquiry. I have been in touch with the source of this antibody and the originator has confirmed that this antibody was in fact raised using full length yeast proteasome 19S subunit S4 and was not a tagged version of the protein. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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      Question

      Customer would like to know the concentration of ab3317. Also, what is the expected band size of the 19S subunit of S4 in S. cerevisiae?

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      Answer

      Thank you for your enquiry. The originator of ab3317 has informed me that the concentration has not been determined and the expected band size in S.cerevisiae is not known to them. If you have any more questions, please contact us again.

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