リコンビナントRabMAb

Anti-Prosurfactant Protein C 抗体 [EPR19839] - BSA and Azide free (ab271991)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
  • Immunohistochemistry (Frozen sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
  • Immunoprecipitation - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
  • Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19839] to Prosurfactant Protein C - BSA and Azide free
  • Suitable for: IP, IHC-Fr, IHC-P, WB
  • Reacts with: Mouse

製品の概要

  • 製品名

    Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free
    Prosurfactant Protein C 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR19839] to Prosurfactant Protein C - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    IP
    Mouse
    See all applications and species data

  • 免疫原

    This product was produced with the following immunogens:
    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse lung lysate .IHC-P: Mouse lung tissue. IHC-Fr: Mouse lung tissue. IP: Mouse lung lysate.

  • 特記事項

    ab271991 is the carrier-free version of ab211326. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-Fr
Mouse
IHC-P
Mouse
IP
Mouse
アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).

 
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
特記事項
IP
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).

 
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.

ターゲット情報

  • 機能

    Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.

  • 関連疾患

    Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
    Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'.

  • 配列類似性

    Contains 1 BRICHOS domain.

  • 細胞内局在

    Secreted > extracellular space > surface film.
  • Information by UniProt

  • 参照データベース

    • 別名

      • BRICD6 antibody
      • BRICHOS domain containing 6 antibody
      • PSP C antibody
      • PSPC antibody
      • PSPC_HUMAN antibody
      • Pulmonary surfactant apoprotein 2 antibody
      • Pulmonary surfactant apoprotein PSP C antibody
      • pulmonary surfactant apoprotein-2 SP-C antibody
      • Pulmonary surfactant associated protein C antibody
      • Pulmonary surfactant associated proteolipid SPL pVal antibody
      • Pulmonary surfactant associated proteolipid SPL(Val) antibody
      • Pulmonary surfactant protein SP5 antibody
      • Pulmonary surfactant-associated protein C antibody
      • Pulmonary surfactant-associated proteolipid SPL(Val) antibody
      • SFTP 2 antibody
      • SFTP2 antibody
      • SFTPC antibody
      • SFTPC surfactant pulmonary associated protein C antibody
      • SMDP2 antibody
      • SP 5 antibody
      • SP C antibody
      • SP-C antibody
      • SP5 antibody
      • SPC antibody
      • Surfactant associated protein pulmonary 2 antibody
      • Surfactant protein c antibody
      • Surfactant proteolipid SPL-pVal antibody
      • Surfactant pulmonary associated protein C antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)

      Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Prosurfactant Protein C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID: 15186480].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Prosurfactant Protein C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Negative control: no staining on mouse spleen. [PMID: 15186480]. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).

    • Immunohistochemistry (Frozen sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
      Immunohistochemistry (Frozen sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Prosurfactant Protein C with ab211326  at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID: 23967208].

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).

    • Immunoprecipitation - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
      Immunoprecipitation - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)

      SP-C was immunoprecipitated from 0.35 mg of mouse lung lysate with ab211326 at 1/40 dilution.

      Western blot was performed from the immunoprecipitate using ab211326 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

      Lane 1: Mouse lung lysate, 10ug (Input).

      Lane 2: ab211326 IP in mouse lung lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211326 in mouse lung lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 5 seconds.

      The banding pattern represents pro- and mature forms of SFTPC.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).
    • Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)
      Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab271991 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab271991 は論文での使用が確認できていません。

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