Anti-Prosurfactant Protein C 抗体 [EPR19839-8] (ab270521)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19839-8] to Prosurfactant Protein C
- Suitable for: IHC-P, WB, IHC-Fr, IP
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-Prosurfactant Protein C antibody [EPR19839-8]
Prosurfactant Protein C 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR19839-8] to Prosurfactant Protein C -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IHC-Fr, IPmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Mouse lung tissue lysate. IHC-P: Mouse lung tissue. IHC-Fr: Mouse lung tissue. IP: Mouse lung tissue lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR19839-8 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab270521の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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IHC-Fr |
1/100.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
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IP |
1/30.
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特記事項 |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
IHC-Fr
1/100. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
IP
1/30. |
ターゲット情報
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機能
Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. -
関連疾患
Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'. -
配列類似性
Contains 1 BRICHOS domain. -
細胞内局在
Secreted > extracellular space > surface film. - Information by UniProt
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参照データベース
- Entrez Gene: 20389 Mouse
- SwissProt: P21841 Mouse
- Unigene: 24040 Mouse
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別名
- BRICD6 antibody
- BRICHOS domain containing 6 antibody
- PSP C antibody
see all
画像
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Anti-Prosurfactant Protein C antibody [EPR19839-8] (ab270521) at 1/1000 dilution + Mouse lung tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking and diluting buffer and concentration: This target has a precursor which contains an exceedingly hybrophobic region of 34 amino acids that comprises most of the mature Spftc, and a unique poly-valine domain. The staining pattern of our antibody is similar with published paper (PMID: 20378731 and PMID: 22461427). 15 seconds Exposure time:
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Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Prosurfactant Protein C with ab270521 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Type II alveolar cells in mouse lung. The section was incubated with ab270521 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung tissue labeling Prosurfactant Protein C with ab270521 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on mouse lung is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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Prosurfactant Protein C was immunoprecipitated from 0.35 mg Mouse lung tissue lysate 1with ab270521 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab270521 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse lung tissue lysate 10 ug.
Lane 2: ab270521 IP in Mouse lung tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270521 in mouse lung tissue lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 6 seconds.
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Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Prosurfactant Protein C with ab270521 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The Pulmonary surfactant-associated protein C is a peptide secreted by the alveolar type II pneumocytes of the lung (PMID: 16769199). Negative control: No staining on mouse spleen. The section was incubated with ab270521 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab270521 は論文での使用が確認できていません。