Anti-Prostate Specific Antigen 抗体 [SP317] (ab224799)
Key features and details
- Rabbit monoclonal [SP317] to Prostate Specific Antigen
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Prostate Specific Antigen antibody [SP317]
Prostate Specific Antigen 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP317] to Prostate Specific Antigen -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Prostate Specific Antigen (internal sequence). The exact sequence is proprietary.
Database link: P07288 -
ポジティブ・コントロール
- WB: LNCaP cell lysate. IHC-P: Human prostate tissue.
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
Purified from TCS by protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
SP317 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab224799の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/100. Predicted molecular weight: 29 kDa.
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IHC-P |
1/100.
Perform heat mediated antigen retrieval with EDTA pH 8.0 buffer before commencing with IHC staining protocol. |
特記事項 |
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WB
1/100. Predicted molecular weight: 29 kDa. |
IHC-P
1/100. Perform heat mediated antigen retrieval with EDTA pH 8.0 buffer before commencing with IHC staining protocol. |
ターゲット情報
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機能
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. -
配列類似性
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 354 Human
- Omim: 176820 Human
- SwissProt: P07288 Human
- Unigene: 171995 Human
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別名
- antigen, prostate-specific antibody
- APS antibody
- Gamma seminoprotein antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate tissue sections labeling Prostate Specific Antigen with ab224799 at 1/100 dilution (3.83 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on the human prostate, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224799 for 10 mins at room temperature. -
Anti-Prostate Specific Antigen antibody [SP317] (ab224799) at 1/100 dilution + LNCaP (human prostate cancer cell line) cell lysate
Predicted band size: 29 kDa -
Formalin-fixed, paraffin-embedded human prostate tissue stained for Prostate Specific Antigen using ab224799 at 1/100 dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab224799 は 1 報の論文で使用されています。
- Cheaito K et al. Establishment and characterization of prostate organoids from treatment-naïve patients with prostate cancer. Oncol Lett 23:6 (2022). PubMed: 34820005