製品の概要

  • 製品名
    Anti-Prostate Specific Antigen antibody [8301]
    Prostate Specific Antigen 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [8301] to Prostate Specific Antigen
  • 由来種
    Mouse
  • 特異性
    Human prostate-specific antigen - 100% PSA/alpha-1-ACT-complex - 59% Kallikrein - <0.04% PAP - <0.04% Cross-reactivity values have been calculated on weight/weight basis using 125-I-labelled human human prostate-specific antigen in assay. They can vary because of the presence of antigen or related impurities in protein preparations used for cross reactivity assays. Affinity constant: 1x1011 l/mol human prostate-specific antigen.
  • アプリケーション
    適用あり: ELISA, RIA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    native protein (purified), corresponding to amino acids 158-163 of Human Prostate Specific Antigen

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
RIA
IHC-P
  • 追加情報
    ELISA: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependent dilution.
    RIA: Use at an assay dependant concentration.
    Nanowires array: 1/50 - 1/500.
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
    • 配列類似性
      Belongs to the peptidase S1 family. Kallikrein subfamily.
      Contains 1 peptidase S1 domain.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • antigen, prostate-specific antibody
      • APS antibody
      • Gamma seminoprotein antibody
      • Gamma-seminoprotein antibody
      • hK3 antibody
      • Kallikrein 3 antibody
      • Kallikrein related peptidase 3 antibody
      • Kallikrein-3 antibody
      • KLK 3 antibody
      • KLK2A1 antibody
      • Klk3 antibody
      • KLK3_HUMAN antibody
      • P-30 antigen antibody
      • P30 antigen antibody
      • Prostate-specific antigen antibody
      • Psa antibody
      • Semenogelase antibody
      • Seminin antibody
      see all

    画像

    • Human normal prostate. Staining is localised as cytoplasmic and secreted. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system, at room temperature: sections were rehydrated and antigen retrieved with buffers (citrate pH 6.1) in a PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    プロトコール

    参考文献

    This product has been referenced in:
    • Shu SK  et al. Phosphorylation and activation of androgen receptor by Aurora-A. J Biol Chem 285:33045-53 (2010). WB ; Human . Read more (PubMed: 20713353) »
    • Kim D  et al. Microarray-based multiplexed scanometric immunoassay for protein cancer markers using gold nanoparticle probes. Anal Chem 81:9183-7 (2009). Read more (PubMed: 19874062) »
    See all 4 Publications for this product

    レビューと Q&A

    1-10 of 19 Abreviews or Q&A

    Application
    ELISA
    Sample
    Human Purified protein (Prostate Specific Antigen from human semen(Purity)
    Specification
    Prostate Specific Antigen from human semen(Purity
    Type
    Sandwich (Capture)

    Mr. Chang Su Jeon

    Verified customer

    投稿 Jul 27 2015

    Question
    Answer

    Thank you for contacting us.

    ab403 - anti-prostate specific antigen antibody [8301] can recognize both free PSA and PSA in the form of a complex.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Answer

    Thank you for your reply.

    I am glad you found the reference useful, I am sorry it took a little while to get to you. Unfortunately, the experiment you are planning would not be covered by the Abpromise guarantee. This is because we have not ourselves tested the antibody in such an application (and have not recieved any information from our customers to support it) and would not therefore be able to guarantee how the antibody might perform.

    However, as we are not able to guarantee it, I can offer you a testing discount. The way this scheme works is that if you purchase the antibody as normal, test it in your binding assay and share the results with us through an Abreview (no matter whether the results are positive or negative), you would then be entitled to the value of ab403 off any products in our catalogue.

    More information on this offer can be found from the following link:

    www.abcam.com/abtrial

    The only limitation to this offer is that the antibody needs to be purchased, the Abreview submitted and the discount code used on a new order within a 4 month period.

    If you would like to participate in this scheme please do let me know as a discount code needs to be raised before the purchase of ab403.

    I hope this information has been of some help. I look forward to receiving your reply.

    Read More

    Question
    Answer

    I am sorry for the delay in getting back to you.

    The epitope mapping performed with the Anti-Prostate Specific Antigen antibody (ab403) can be found in the reference I have attached to this email.

    The information can be found on page 7. The ab403 is of the clone number 8301 (ISOBM code #257) and has been mapped to the epitope group 3a. This is the region 158-163 of human Prostate Specific Antigen.

    Please note that we currently have the clone number set to PSA1 but this will be updated. The clone number should be 8301.

    I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

    Read More

    Question
    Answer

    Thank you for your telephone enquiry on Friday.

    As discussed on the telephone, I am sorry that as far as we are aware, ab403 PSA antibody has never been tested with samples from cow. All tested species covered by the guarantee are stated on the online datasheets, and these are updated as soon as any new information is brought to our attention.

    I wanted to check the alignment of the immunogen with the bovine sequence. However, I am sorry to confirm that regrettably I have been unable to find the bovine sequence in the protein database sites. I would appreciate if it is possible for you to send the bovine amino acid sequence and I will be happy to do an alignment for you so we can predict whether or not this antibody may wok on cow samples.

    If the alignment is high (> 70%), we would be pleased to provide our testing discount program. This works as follows:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab403 in cow. I can then check the alignment. If the alignment is high, I can then send a discount code. This code must be issued before purchasing ab403 so please wait for my reply before ordering.

    If we are able to provide a discount code you would then proceed as follows:

    2. Purchase ab403 either by phone, fax, or online (https://www.abcam.com). This order is paid at full price.

    3. Test the antibody in cow.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab403 turns out to be unsuitable for cow, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact me with the further requested details.

    Read More

    Answer

    I am sorry for the delay in getting back to you.

    I have found an antibody which may work in your experiments which I am able to share the immunogen information with you. The anti-Prostate Specific Antigen antibody [PSA1] antibody ab403 was raised against the native protein. However, epitope mapping has been performed to establish that the antibody binds to a region between residues 158-163 of the human protein.

    You may therefore be able to synthesise this region of the protein to perform your experiment. Do you think this antibody might be suitable for your experiment? I would like to point out that we have not tried what you are hoping to use the antibody for and would therefore not be able to predict how it might perform.

    I am waiting on a reference which details of how the epitope mapping was performed which may provide you with some useful information about different peptides used to probe the antibody with. Unfortunately, the person who has this information is not available until the middle of next week. Once I have this information I will pass it on to you.

    I hope this information has been of help. If you have any further questions, please do not hesitate to let me know.

    Read More

    Answer

    Thank you for contacting us.

    All of the antibodies you have chosen are appropriate for the experiments you are hoping to perform. All of the antibodies have been raised against the human protein but the cross reactivity with the mouse protein has not been ascertained and I cannot therefore say whether they will also be able to detect the mouse form of the protein.

    There are a few antibodies which may be worth considering as they may provide more experiments for the cost. All antibodies require optimisation before achieving the optimum results and it may therefore be that you will need to use more or less antibody than advised on the datasheets. The dilutions stated on the datasheets are guidelines only and will depend on a number of different factors.

    For the anti-AMACR antibody you may want to consider ab63340. This is amouse monoclonal, provided at a volume of 100 ul at the same price as ab12498 but has been shown to be usable at a dilution of1/500 to 1/2000 compared to 1/25 to 1/50 for ab12498 (500 uL provided).

    For the anti-Androgen Receptor antibody you may want to consider ab9474 (250 uL with a recommended dilution of 1/25-1/50). This should provide roughly the same number of experiments as ab47569 (100 uL with a recommended dilution of 1/50-1/100).

    For the anti-Mitochondria antibody, you may want to consider ab3298. This is provided at a volume of 500 uL with a recommended dilution of 1/500-1/1000 compared to ab92824 for which 100 uL is provided at a recommended dilution of 1/1000.

    For the anti-Prostate Specific Antigen antibody I was unable to identify another antibody which would provide you with more experiments.

    I hope this information has been of help. If you have any further questions please do not hesitate to contact us again.

    Read More

    Answer

    Thank you for contacting us.

    Indeed, I can confirm that PBS as dilution buffer is suitable for all three antibodies and the protein.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question
    Answer

    Thank you for your enquiry. The affinity constant was determined by RIA. In brief, according to the lab that performed this characterization: "Dilution series of antibody ab403 has been prepared and all dilutions have been incubated with 125 I (iodine)labeled PSA antigen. Bound antibodies have been separated with PEG precipitation and counts have been measured. Amount of free antibodies has been calculated by using total max of the test run. Affinity curve has been formed from values of bound/free versus bound and then the coefficient has been calculated using slope of the curve and molecular weight of the antigen." More detailed method descriptions can be found in the literature or on the internet. I recommend a book from the Practical Approach series: Immunoassays, edited by J. P. Gosling, from Oxford University Press, 2000. I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Answer

    Thank you for your patience in this matter. I have received a reply from the originator of ab403 (PSA antibody). The pI value of this product is 6,0 - 6,7. The assay method is PhastSystem, PhastGel IEF 3-9. I hope this information is useful for you.

    Read More

    1-10 of 19 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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