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  1. Link

    prostaglandin-e2-antibody-ab2318.pdf

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Signal Transduction Signaling Pathway Lipid Signaling Prostaglandins
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Anti-Prostaglandin E2 抗体 (ab2318)

  • Datasheet
  • SDS
Reviews (4)Q&A (14)References (24)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Rabbit polyclonal to Prostaglandin E2
  • Suitable for: ELISA
  • Reacts with: Species independent
  • Isotype: IgG

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

製品の概要

  • 製品名

    Anti-Prostaglandin E2 antibody
    Prostaglandin E2 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Prostaglandin E2
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ELISAmore details
  • 種交差性

    交差種: Species independent
  • 免疫原

    Highly pure Prostaglandin E2 conjugated to bovine thyroglobulin

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • 精製度

    Whole antiserum
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Prostaglandins

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

Our Abpromise guarantee covers the use of ab2318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000 - 1/100000.

ターゲット情報

  • 関連性

    Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis.
  • 別名

    • PG E2 antibody
    • PGE 2 antibody
    • PGE2 antibody
    • ProstaglandinE2 antibody

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (24)

    ab2318 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2318 は 24 報の論文で使用されています。

    • Sajiki Y  et al. Prostaglandin E2-Induced Immune Exhaustion and Enhancement of Antiviral Effects by Anti-PD-L1 Antibody Combined with COX-2 Inhibitor in Bovine Leukemia Virus Infection. J Immunol 203:1313-1324 (2019). PubMed: 31366713
    • Chu TH  et al. Hepatoma-derived growth factor participates in Helicobacter Pylori-induced neutrophils recruitment, gastritis and gastric carcinogenesis. Oncogene 38:6461-6477 (2019). PubMed: 31332288
    • Barletta ABF  et al. Mosquito Midgut Prostaglandin Release Establishes Systemic Immune Priming. iScience 19:54-62 (2019). PubMed: 31351392
    • Ni S  et al. Sensory innervation in porous endplates by Netrin-1 from osteoclasts mediates PGE2-induced spinal hypersensitivity in mice. Nat Commun 10:5643 (2019). PubMed: 31822662
    • Yokoi E  et al. The role of myeloid-derived suppressor cells in endometrial cancer displaying systemic inflammatory response: clinical and preclinical investigations. Oncoimmunology 8:e1662708 (2019). PubMed: 31741758
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 18 Abreviews or Q&A

    Question

    I am interested in neutralizing human PGE2 in in vitro cultures, and I'm having troubles finding a suitable antibody for this.On your website, I have found some anti-PGE2 antibodies that react with human PGE2, but neutralization in cultures is not listed as a recommended application. I have also found this issue addressed in one of your scientific support FAQs to the antibody with the catalogue number 'ab2318' but the answer was not very conclusive (you do not recommed use of any of these antibodies in culture due to the presenc of sodium azide, but I could find no follow-up entries as to whether the sodium azide can be successfully removed).



    Since this entry dates back to 2006, I was wondering whether you have since made any more experience with these PGE2 antibodies, or whether you have any new products that I could use for my human in vitro neutralization studies.



    I would be very grateful for your help.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 14 2012

    Answer

    Thank you very much for your interest in ab2318 Anti-Prostaglandin E2 antibody.

    To our knowledge, ab2318 has still not been tested in a cell culture assay due to the sodium azide. As my collegue suggest it is possible to extract the chemical via a dialysis step to purify the antibody or to use our purification kit, whichwouldpurify this whole serum antibody on top of this. The purification kit can be found here:

    https://www.abcam.com/index.html?datasheet=109208 (or use the following: https://www.abcam.com/index.html?datasheet=109208).

    As the application hasn't been tested, I can offer a discount off a future purchase if you buy ab2318 now, test it in cell culture assay and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab2318 in cell culture assay. I will then send a discount code. This code must be issued before purchasing ab2318 so please wait for my reply before ordering.

    2. Purchase ab2318 either by phone, fax, or online (www.abcam.com).

    3. Test it in cell culture assay.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab2318 turns out to be unsuitable for cell culture assay, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Mar 14 2012

    Western blot abreview for Anti-Prostaglandin E2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Caco-2 cell)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    40 µg
    Treatment
    25 nM EGF for 0, 4, 8, 24hrs
    Specification
    Caco-2 cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 16 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Prostaglandin E2 antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: (10 mM Sodium citrate, 0.05% Tween 20, pH 6.0)
    Permeabilization
    Yes - 0.5% Tween 20
    Specification
    spleen
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 May 27 2016

    Western blot abreview for Anti-Prostaglandin E2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Non-reduced Denaturing (10 % SDS polyacylamide gel)
    Sample
    Dog Cell lysate - whole cell (adipose tissue derived mesenchymal stem cell)
    Specification
    adipose tissue derived mesenchymal stem cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Dr. Seunghoon Lee

    Verified customer

    投稿 Apr 09 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Prostaglandin E2 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Sample
    Mouse Tissue sections (mouse skin)
    Specification
    mouse skin
    Permeabilization
    Yes - Tween-20
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 22 2014

    Question

    Does it only recognize PGE2? How about PEG2 metabolite like 13,14-dihydro-15-keto PGE2.?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 14 2014

    Answer

    Unfortunately, we do not have information regarding cross reactivity of ab2318 to a PEG2 metabolite like 13,14-dihydro-15-keto PGE2.

    Read More

    Jeremy Kasanov

    Abcam Scientific Support

    Answered on Nov 14 2014

    Question

    Can you please advise if this customer would be eligible for a testing discount;

    ii) ab2318

    iii)we would like to try it on Immunoprecipitation (may use it for ELISA and Western blot eventually , but not a priority at this stage)

    iv)our samples are human

    v) tissue extracts and secreted PGs into culture medium

    Read More

    Abcam community

    Verified customer

    Asked on Jan 23 2013

    Answer

    DISCOUNT CODE: XXXXXXX
    Expiration date: 20-05-2013

    I am very pleased to hear you would like to accept our offer and test ab2318 in IP. This code will give you 100% off your next order before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

    Read More

    Abcam Scientific Support

    Answered on Jan 23 2013

    Question

    Dear Sir or Madam,

    is your product „ Anti-Prostaglandin E2 antibody (ab2318)” available without azide as well?

    Thank you very much,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 27 2012

    Answer

    Thank you for contacting us and your interest in our products.

    We unfortunately do not have the Anti-Prostaglandin E2 antibody (ab2318) available without sodium azide present.

    We do however have purification kits available from our catalogue which can be used to remove the azide. These can be viewed from the following links:

    https://www.abcam.com/index.html?pageconfig=resource&rid=13489

    I hopethis information has been of help. If you have any further questions, please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    Answered on Jun 27 2012

    Question

    Dear technical support team:

    This customer has highly interested in ab2318 (Anti-Prostaglandin E2 antibody), she would likes to order this antibody and test in ELISA with rabbit sample, would you please help this customer to create a discount code, the information of this customer as follow:





    Name: cll
    Email: xxxxx@yahoo.com.tw

    Institution: CHIAYI CHANG GUNG MEMORIAL HOSPITAL OF THE C.G.M.F





    Could you please help this customer to solve the problem?

    Thanks for your kindly help.



    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Apr 26 2012

    Answer

    Thank you for your inquiry.

    I have to confirm thatab2318 is not eligible for the testing programmewith different species.

    The target Prostaglandin E2 is not a protein but a lipid. This chemical will be the same independently of the species.

    For more information on the structure please follow this link:

    http://en.wikipedia.org/wiki/Prostaglandin_E2


    Your customer should not have any problems detecting Prostaglandin E2 in rabbit samples.

    Please keep in mind that since PGE2 is a lipid, extraction buffers containing detergents (for preparing tissue samples) are not recommended. Mechanical disruption in a buffer such as PBS is recommended instead. For serum and plasma, simple dilution should be sufficient.

    I hope this information is helpful and wish your customers good luck for their experiments.

    Read More

    Abcam Scientific Support

    Answered on Apr 26 2012

    Question

    This customer raises an enquiry about ab47027 (Anti-CGRP antibody), ab106291 (Anti-Substance P antibody), ab14184 (Anti-Substance P antibody [SP-DE4-21]) and ab2318 (Anti-Prostaglandin E2 antibody), this customer wants to conduct wb and ELISA with rabbit sample, and therefore she wants to know what percentage the sequence identity is between the immunogen of these antibodies and rabbit’s sequence. Could you please help this customer to solve these problems? Thanks for your kindly help.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 04 2012

    Answer

    Thank you for contacting us.


    I performed a protein alignment with the target immunogens and the rabbit protein sequences for the antibodies. We recommend that alignment should be over 85% to predict that an antibody will detect in a different species.

    Here are the results, similarities are in %:

    ab47027 Anti-CGRP antibody: 85%

    ab14184 Anti-Substance P antibody [SP-DE4-21]: 100%

    ab106291 Anti-Substance P antibody: 100%

    ab2318: As mentioned in the Scientific Support section, PGE2 is a lipid rather than a protein and is therefore not species specific. There should not be any particular problems using this assay to detect PGE2 from rabbit. Optimization for different sample types will have to be done by the end user. Please keep in mind that since PGE2 is a lipid, extraction buffers containing detergents (for preparing tissue samples) are not recommended. Mechanical disruption in a buffer such as PBS is recommended instead. For serum and plasma, simple dilution should be sufficient.


    I can offer a discount off a future purchase if your customer buys one or more of the antibodiesnow, test it in rabbit and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback.


    If they are interested in this offer, please let me know.

    Read More

    Abcam Scientific Support

    Answered on Apr 04 2012

    1-10 of 18 Abreviews or Q&A

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