製品の概要

  • 製品名
    Anti-Prostaglandin E2 antibody
    Prostaglandin E2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Prostaglandin E2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ELISA, IHC-P, ICCmore details
  • 種交差性
    交差種: Species independent
  • 免疫原

    Highly pure Prostaglandin E2 conjugated to bovine thyroglobulin

  • ポジティブ･コントロール
    • Purified PGE2

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium azide
  • 精製度
    Whole antiserum
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000 - 1/100000.
IHC-P Use at an assay dependent concentration.
ICC Use at an assay dependent concentration. PubMed: 23762835

ターゲット情報

  • 関連性
    Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis.
  • 別名
    • PG E2 antibody
    • PGE 2 antibody
    • PGE2 antibody
    • ProstaglandinE2 antibody

参考文献

This product has been referenced in:
  • Li J  et al. Methyl Salicylate Lactoside Protects Neurons Ameliorating Cognitive Disorder Through Inhibiting Amyloid Beta-Induced Neuroinflammatory Response in Alzheimer's Disease. Front Aging Neurosci 10:85 (2018). Read more (PubMed: 29636677) »
  • Ho ATV  et al. Prostaglandin E2 is essential for efficacious skeletal muscle stem-cell function, augmenting regeneration and strength. Proc Natl Acad Sci U S A 114:6675-6684 (2017). IHC ; Mouse . Read more (PubMed: 28607093) »

See all 12 Publications for this product

ab2318 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

17 reviews or Q&A

10
Votes: Highest First

I am interested in neutralizing human PGE2 in in vitro cultures, and I'm having troubles finding a suitable antibody for this.On your website, I have found some anti-PGE2 antibodies that react with human PGE2, but neutralization in cultures is not list...

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Thank you very much for your interest in ab2318 Anti-Prostaglandin E2 antibody.

To our knowledge, ab2318 has still not been tested in a cell culture assay due to the sodium azide. As my collegue suggest it is possible to extract the chemical...

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Prostaglandin E2 antibody

 Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: (10 mM Sodium citrate, 0.05% Tween 20, pH 6.0)
Permeabilization
Yes - 0.5% Tween 20
Specification
spleen
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
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Username

Abcam user community

Verified customer

投稿 May 27 2016

Western blot abreview for Anti-Prostaglandin E2 antibody

 Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (10 % SDS polyacylamide gel)
Sample
Dog Cell lysate - whole cell (adipose tissue derived mesenchymal stem cell)
Specification
adipose tissue derived mesenchymal stem cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Username

Dr. Seunghoon Lee

Verified customer

投稿 Apr 09 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Prostaglandin E2 antibody

 Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Sample
Mouse Tissue sections (mouse skin)
Specification
mouse skin
Permeabilization
Yes - Tween-20
Fixative
Paraformaldehyde
Read More
abreview image
Username

Abcam user community

Verified customer

投稿 Dec 22 2014

Does it only recognize PGE2? How about PEG2 metabolite like 13,14-dihydro-15-keto PGE2.?

Unfortunately, we do not have information regarding cross reactivity of ab2318 to a PEG2 metabolite like 13,14-dihydro-15-keto PGE2.

Can you please advise if this customer would be eligible for a testing discount;

ii) ab2318

iii)we would like to try it on Immunoprecipitation (may use it for ELISA and Western blot eventually , but not a priority at this stage)
<...

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DISCOUNT CODE: XXXXXXX
Expiration date: 20-05-2013

I am very pleased to hear you would like to accept our offer and test ab2318 in IP. This code will give you 100% off your next order before the expiration date. To redeem this offer, pleas...

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Dear Sir or Madam,

is your product „ Anti-Prostaglandin E2 antibody (ab2318)” available without azide as well?

Thank you very much,

Thank you for contacting us and your interest in our products.

We unfortunately do not have the Anti-Prostaglandin E2 antibody (ab2318) available without sodium azide present.

We do however have purification kits available from our ...

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Dear technical support team:

This customer has highly interested in ab2318 (Anti-Prostaglandin E2 antibody), she would likes to order this antibody and test in ELISA with rabbit sample, would you please help this customer to create a discoun...

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Thank you for your inquiry.

I have to confirm thatab2318 is not eligible for the testing programmewith different species.

The target Prostaglandin E2 is not a protein but a lipid. This chemical will be the same independently of the ...

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This customer raises an enquiry about ab47027 (Anti-CGRP antibody), ab106291 (Anti-Substance P antibody), ab14184 (Anti-Substance P antibody [SP-DE4-21]) and ab2318 (Anti-Prostaglandin E2 antibody), this customer wants to conduct wb and ELISA with rabb...

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Thank you for contacting us.


I performed a protein alignment with the target immunogens and the rabbit protein sequences for the antibodies. We recommend that alignment should be over 85% to predict that an antibody will detect in a di...

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Dear abcam technical support team: This customer would like to order ab2318 (Anti-Prostaglandin E2 antibody), he would likes to order this antibody and test in IHC-P assay with rat sample, would you please help this customer to create a discount code, ...

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DISCOUNT CODE: XXXXX Expiration date: 12/05/2012 I am very pleased to hear you would like to accept our offer and testab2318 in Rat in IHC-P. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submi...

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1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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