Anti-Prostaglandin E Receptor EP2/PTGER2 抗体 [EPR8030(B)] (ab167171)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8030(B)] to Prostaglandin E Receptor EP2/PTGER2
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Cow, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)]
Prostaglandin E Receptor EP2/PTGER2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8030(B)] to Prostaglandin E Receptor EP2/PTGER2 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IFmore details
適用なし: IHC-P -
種交差性
交差種: Mouse, Rat, Cow, Human -
免疫原
Synthetic peptide corresponding to Human Prostaglandin E Receptor EP2/PTGER2 aa 300-400 (C terminal).
Database link: P43116 -
ポジティブ・コントロール
- Human fetal lung, JAR and WI38 cell lysates. This antibody gave a positive result when used in the following methanol fixed cell lines: SV40LT-SMC
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR8030(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- HRP Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (ab203462)
- Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] - BSA and Azide free (ab236779)
- Alexa Fluor® 488 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (ab246777)
- Alexa Fluor® 647 Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (ab246778)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab167171の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/80 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
|
ICC/IF |
1/250 - 1/500.
|
特記事項 |
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Flow Cyt (Intra)
1/80 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
ICC/IF
1/250 - 1/500. |
ターゲット情報
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機能
Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle. -
組織特異性
Placenta and lung. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 282329 Cow
- Entrez Gene: 5732 Human
- Entrez Gene: 19217 Mouse
- Entrez Gene: 100360765 Rat
- Entrez Gene: 81752 Rat
- Omim: 176804 Human
- SwissProt: P43116 Human
- SwissProt: Q62053 Mouse
see all -
別名
- EP2 antibody
- PE2R2_HUMAN antibody
- PGE receptor EP2 subtype antibody
see all
画像
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All lanes : Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (ab167171) at 1/1000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat spleen tissue lysate
Lane 4 : MDBK (cow kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 40 kDaBlocking and diluting buffer: 5% NFDM/TBST
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ab167171 staining Prostaglandin E Receptor EP2/PTGER2 in theWI-38 (Human Fetal Lung Fibroblast) cell lineby intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/80. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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ab167171 staining Prostaglandin E Receptor EP2/PTGER2 in WI-38 (Human Fetal Lung Fibroblast) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab167171 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.
Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) -
All lanes : Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (ab167171) at 1/1000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : WI-38 (human lung) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 40 kDaBlocking and diluting buffer: 5% NFDM/TBST
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ab167171 stained SV40LT-SMC (rat) cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab167171 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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All lanes : Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (ab167171) at 1/1000 dilution
Lane 1 : Human placenta lysates
Lane 2 : Human fetal lung lysates
Lane 3 : JAR cell lysates
Lane 4 : WI38 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 40 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab167171 は 15 報の論文で使用されています。
- Lu Y et al. Bone marrow mesenchymal stem cell-derived exosomes improve renal fibrosis by reducing the polarisation of M1 and M2 macrophages through the activation of EP2 receptors. IET Nanobiotechnol 16:14-24 (2022). PubMed: 34862858
- Huang Q et al. Higher fibrotic content of endometriotic lesions is associated with diminished prostaglandin E2 signaling. Reprod Med Biol 21:e12423 (2022). PubMed: 34938147
- Huang Q et al. Changing prostaglandin E2 (PGE2) signaling during lesional progression and exacerbation of endometriosis by inhibition of PGE2 receptor EP2 and EP4. Reprod Med Biol 21:e12426 (2022). PubMed: 34938150
- Jiang W et al. PGE2 activates EP4 in subchondral bone osteoclasts to regulate osteoarthritis. Bone Res 10:27 (2022). PubMed: 35260562
- Varvel NH et al. Peripheral Myeloid Cell EP2 Activation Contributes to the Deleterious Consequences of Status Epilepticus. J Neurosci 41:1105-1117 (2021). PubMed: 33293358