Anti-Prostaglandin D Synthase (Lipocalin)/PDS 抗体 [EP12357] (ab182141)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP12357] to Prostaglandin D Synthase (Lipocalin)/PDS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357]
Prostaglandin D Synthase (Lipocalin)/PDS 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP12357] to Prostaglandin D Synthase (Lipocalin)/PDS -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab188544) -
ポジティブ・コントロール
- WB:HepG2, SW480, and THP-1 whole cell lysate IHC-P: Human glioma ICC/IF: HepG2 cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP12357 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab182141の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 21 kDa).Can be blocked with Prostaglandin D Synthase (Lipocalin)/PDS peptide (ab188544).
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IHC-P |
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/200.
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特記事項 |
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WB
1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 21 kDa).Can be blocked with Prostaglandin D Synthase (Lipocalin)/PDS peptide (ab188544). |
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/200. |
ターゲット情報
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機能
Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system. -
組織特異性
Abundant in the brain and CNS, where it is expressed in tissues of the blood-brain barrier and secreted into the cerebro-spinal fluid. Abundantly expressed in the heart. In the male reproductive system, it is expressed in the testis, epididymis and prostate, and is secreted into the seminal fluid. Expressed in the eye and secreted into the aqueous humor. Lower levels detected in various tissue fluids such as serum, normal urine, ascitic fluid and tear fluid. Also found in a number of other organs including ovary, fimbriae of the fallopian tubes, kidney, leukocytes. -
配列類似性
Belongs to the calycin superfamily. Lipocalin family. -
発生段階
Expression in the amniotic fluid increases dramatically during weeks 12 to 25 of pregnancy. Levels decrease slowly after 25 weeks. -
ドメイン
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
翻訳後修飾
Both N-glycosylation recognition sites are almost quantitatively occupied by N-glycans of the biantennary complex type, with a considerable proportion of structures bearing a bisecting GlcNAc. Agalacto structure as well as sialylated and nonsialylated oligosaccharides bearing alpha2-3- and/or alpha2-6-linked NeuNAc are present. -
細胞内局在
Rough endoplasmic reticulum. Nucleus membrane. Golgi apparatus. Cytoplasm > perinuclear region. Secreted. Detected on rough endoplasmic reticulum of arachnoid and menigioma cells. Localized to the nuclear envelope, Golgi apparatus, secretory vesicles and spherical cytoplasmic structures in arachnoid trabecular cells, and to circular cytoplasmic structures in meningeal macrophages and perivascular microglial cells. In oligodendrocytes, localized to the rough endoplasmic reticulum and nuclear envelope. In retinal pigment epithelial cells, localized to distinct cytoplasmic domains including the perinuclear region. Also secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 5730 Human
- Omim: 176803 Human
- SwissProt: P41222 Human
- Unigene: 446429 Human
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別名
- Beta trace protein antibody
- Beta-trace protein antibody
- Cerebrin 28 antibody
see all
画像
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All lanes : Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141) at 1/10000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 15 µg
Lane 2 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Exposure time: 60 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Primary and secondary antibodies: incubated for 1h at room temperature.
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The human glioma paraffin-embedded sections were Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins and labeling ab182141 at 1/200 dilution for 30 mins at room temperature, then followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
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HepG2 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab182141 at 1/200 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Rabbit secondary antibody (ab150081) at 1/1000 dilution at RT for 45 min. ab195889 AlexaFluor® 594-conjugated Anti-alpha Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab182141 overnight at 4° C. Nucleus were visualized using DAPI.
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Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141) at 1/1000 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted? -
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Prostaglandin D Synthase (Lipocalin)/PDS with ab182141 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counterstained with DAPI.
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Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Prostaglandin D Synthase (Lipocalin)/PDS with ab182141 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141) at 1/1000 dilution + Human fetal brain lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 21 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (9)
ab182141 は 9 報の論文で使用されています。
- Diao B et al. Human kidney is a target for novel severe acute respiratory syndrome coronavirus 2 infection. Nat Commun 12:2506 (2021). PubMed: 33947851
- Xu W et al. Dexamethasone Suppresses Radicular Pain Through Targeting the L-PGDS/PI3K/Akt Pathway in Rats With Lumbar Disc Herniation. Pain Pract 21:64-74 (2021). PubMed: 32640501
- Wu S et al. The prostaglandin synthases, COX-2 and L-PGDS, mediate prostate hyperplasia induced by low-dose bisphenol A. Sci Rep 10:13108 (2020). PubMed: 32753632
- Wegmann R et al. CellSIUS provides sensitive and specific detection of rare cell populations from complex single-cell RNA-seq data. Genome Biol 20:142 (2019). PubMed: 31315641
- Liu YR et al. ER-depletion lowering the 'hypothalamus-uterus-kidney' axis functions by perturbing the renal ERß/Ptgds signalling pathway. Aging (Albany NY) 11:9500-9529 (2019). PubMed: 31708494
- Chai D et al. Multiple sevoflurane exposures during pregnancy inhibit neuronal migration by upregulating prostaglandin D2 synthase. Int J Dev Neurosci 78:77-82 (2019). PubMed: 31499143
- Li X et al. Endogenous ET-1 promotes ANP secretion through activation of COX2-L-PGDS-PPAR? signaling in hypoxic beating rat atria. Peptides 122:170150 (2019). PubMed: 31541683
- Corwin C et al. Prostaglandin D2/J2 signaling pathway in a rat model of neuroinflammation displaying progressive parkinsonian-like pathology: potential novel therapeutic targets. J Neuroinflammation 15:272 (2018). PubMed: 30236122
- Isensee J et al. Subgroup-elimination transcriptomics identifies signaling proteins that define subclasses of TRPV1-positive neurons and a novel paracrine circuit. PLoS One 9:e115731 (2014). PubMed: 25551770