Key features and details
- Mouse monoclonal [U5] to Prolactin Receptor/PRL-R
- Suitable for: Flow Cyt, IHC-P, ICC/IF
- Reacts with: Rat, Sheep, Rabbit, Horse, Human, Pig
- Isotype: IgG1
製品名Anti-Prolactin Receptor/PRL-R antibody [U5]
Prolactin Receptor/PRL-R 一次抗体 製品一覧
製品の詳細Mouse monoclonal [U5] to Prolactin Receptor/PRL-R
特異性Detects prolactin (PRL) receptor.
アプリケーション適用あり: Flow Cyt, IHC-P, ICC/IFmore details
種交差性交差種: Rat, Sheep, Rabbit, Horse, Human, Pig
交差が予測される動物種: Non human primates
Other Immunogen Type corresponding to Rat Prolactin Receptor/PRL-R. Purified rat liver PRL receptor.
エピトープThis antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain.
Previously labelled as Prolactin Receptor.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.05% Sodium azide
Concentration information loading...
精製度Protein G purified
一次抗体 備考Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
Our Abpromise guarantee covers the use of ab2772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration.|
機能This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
組織特異性Expressed in breast, placenta, kidney, liver and pancreas.
配列類似性Belongs to the type I cytokine receptor family. Type 1 subfamily.
Contains 2 fibronectin type-III domains.
ドメインThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
細胞内局在Secreted and Membrane.
- Information by UniProt
- AI987712 antibody
- CLONE SPM213 antibody
- CPRLP antibody
Mammary gland tissue from lactating light horse mare was incubated with ab2772 at 1:100 for 1 hour at 23°C and for 24 hours at 4°C. A biotin-conjugated goat anti-mouse IgG was used as the secondary antibody (23°C for 1 hour) .Development using avidin-biotin complex 23°C for 1 hour plus 3,3-diaminobenzadine for 15 min and counterstained with haematoxylin. Antigen retrieval was facilitated using steaming at 95°C for 20 min in Trizma® buffer (pH 10.0) . Blocking endogenous peroxidase performed with 0.3% H2O2 and MeOH (20 min at 23°C). To decrease non-specific staining, tissue was pre-incubated in normal goat serum for 30 min at 23°C.
Immunocytochemistry/Immunofluorescence analysis of Prolactin Receptor/PRL-R shows staining in A2058 cells. Prolactin Receptor/PRL-R (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2772 (1:20) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
Overlay histogram showing MCF-7 cells stained with ab2772 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2772, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Immunocytochemistry/Immunofluorescence analysis of Prolactin Receptor/PRL-R shows staining in HepG2 cells. Prolactin Receptor/PRL-R (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2772 (1:20) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
Immunocytochemistry/Immunofluorescence analysis of Prolactin Receptor/PRL-R shows staining in MCF-7 cells. Prolactin Receptor/PRL-R (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2772 (1:20) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
ab2772 staining the Prolactin Receptor/PRL-R in Human salivary gland tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was not performed. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-mouse polyclonal (1/200) was used as the secondary antibody.
ab2772 は 16 報の論文で使用されています。
- Qin R et al. Mst1 deletion reduces hyperglycemia-mediated vascular dysfunction via attenuating mitochondrial fission and modulating the JNK signaling pathway. J Cell Physiol 235:294-303 (2020). PubMed: 31206688
- Morar II et al. Immunohistochemical study of psoriatic plaques and perilesional skin in psoriasis vulgaris patients: A pilot study. Exp Ther Med 18:888-894 (2019). PubMed: 31384319
- Zhu W et al. Effect of docosahexaenoic acid on traumatic brain injury in rats. Exp Ther Med 14:4411-4416 (2017). PubMed: 29075341
- Xiao F et al. Central prolactin receptors (PRLRs) regulate hepatic insulin sensitivity in mice via signal transducer and activator of transcription 5 (STAT5) and the vagus nerve. Diabetologia 57:2136-44 (2014). PubMed: 25064125
- Bridges RS et al. Reproductive experience alters prolactin receptor expression in mammary and hepatic tissues in female rats. Biol Reprod 85:340-6 (2011). WB ; Rat . PubMed: 21508351