Anti-Prolactin Receptor/PRL-R 抗体 [EPR7184(2)] (ab170935)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7184(2)] to Prolactin Receptor/PRL-R
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)]
Prolactin Receptor/PRL-R 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7184(2)] to Prolactin Receptor/PRL-R -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human Prolactin Receptor/PRL-R aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P16471 -
ポジティブ・コントロール
- T47-D, (B) A431 and (C) Jurkat whole cell lysate (ab7899), Human breast carcinoma & kidney tissue, Jurkat cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7184(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab170935の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 70 kDa.
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IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab170935 shows background nuclear staining on mouse colon,pancreas and lung. |
ICC/IF |
1/100 - 1/250.
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特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 70 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. ab170935 shows background nuclear staining on mouse colon,pancreas and lung. |
ICC/IF
1/100 - 1/250. |
ターゲット情報
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機能
This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling. -
組織特異性
Expressed in breast, placenta, kidney, liver and pancreas. -
配列類似性
Belongs to the type I cytokine receptor family. Type 1 subfamily.
Contains 2 fibronectin type-III domains. -
ドメイン
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
細胞内局在
Secreted and Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5618 Human
- Entrez Gene: 19116 Mouse
- Entrez Gene: 24684 Rat
- Omim: 176761 Human
- SwissProt: P16471 Human
- SwissProt: Q08501 Mouse
- SwissProt: P05710 Rat
- Unigene: 368587 Human
see all -
別名
- AI987712 antibody
- CLONE SPM213 antibody
- CPRLP antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Prolactin Receptor / PRL-R with ab170935 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)] (ab170935) at 1/1000 dilution
Lane 1 : T47-D cell lysates
Lane 2 : A431 cell lysates
Lane 3 : Jurkat cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 70 kDa -
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Prolactin Receptor / PRL-R with ab170935 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescence analysis of Jurkat cells labeling Prolactin Receptor / PRL-R with ab170935 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab170935 は 10 報の論文で使用されています。
- Wu Z et al. Energy deprivation-induced AMPK activation inhibits milk synthesis by targeting PrlR and PGC-1α. Cell Commun Signal 20:25 (2022). PubMed: 35248054
- Jüngert K et al. Prolactin Inducible Protein, but Not Prolactin, Is Present in Human Tears, Is Involved in Tear Film Quality, and Influences Evaporative Dry Eye Disease. Front Med (Lausanne) 9:892831 (2022). PubMed: 35847789
- Shao C et al. Hormone-Responsive BMP Signaling Expands Myoepithelial Cell Lineages and Prevents Alveolar Precocity in Mammary Gland. Front Cell Dev Biol 9:691050 (2021). PubMed: 34336839
- Abu Quora HA et al. Microenvironment of mammary fat pads affected the characteristics of the tumors derived from the induced cancer stem cells. Am J Cancer Res 11:3475-3495 (2021). PubMed: 34354856
- Vergneau-Grosset C et al. Altered prolactin and androgen receptors expression in companion rat benign mammary tumours. Vet Comp Oncol 19:213-221 (2021). PubMed: 33191604
- Alkharusi A et al. Connections between prolactin and ovarian cancer. PLoS One 16:e0255701 (2021). PubMed: 34358244
- Horiguchi K et al. Expression and functions of cluster of differentiation 9 and 81 in rat mammary epithelial cells. J Reprod Dev 66:515-522 (2020). PubMed: 32830152
- Zhang P et al. Prolactin improves hepatic steatosis via CD36 pathway. J Hepatol 68:1247-1255 (2018). IHC-P ; Human . PubMed: 29452209
- Gao Z et al. Expression of Stem Cell Markers and Dopamine D2 Receptors in Human and Rat Prolactinomas. Med Sci Monit 23:1827-1833 (2017). IHC . PubMed: 28411401
- Yu L et al. MFGE8 regulates TGF-ß-induced epithelial mesenchymal transition in endometrial epithelial cells in vitro. Reproduction 152:225-33 (2016). ICC/IF ; Human . PubMed: 27340235