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RabMAb

Anti-Prohibitin 抗体 [EP2804Y] - Mitochondrial Marker (ab75771)

製品の概要

  • 製品名
    Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker
    Prohibitin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2804Y] to Prohibitin - Mitochondrial Marker
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-Fr, WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Prohibitin aa 150-250. The exact sequence is proprietary.

  • ポジティブ・コントロール
    • WB: MCF-7, PC-12, A431 and Ramos cell lysate; IHC-P: human hepatocellular carcinoma tissue, mouse kidney tissue, mouse endometrium tissue, mouse liver tissue, rat liver tissue and rat stomach tissue; ICC/IF: Hela and Neuro-2a cells. IP: MCF7
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 22125622
WB 1/500 - 1/1000. Predicted molecular weight: 30 kDa.
IP 1/20.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.

ターゲット情報

  • 機能
    Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
  • 組織特異性
    Widely expressed in different tissues.
  • 配列類似性
    Belongs to the prohibitin family.
  • 発生段階
    Levels of expression in fibroblasts decrease heterogeneously during cellular aging.
  • 細胞内局在
    Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Epididymis luminal protein 215 antibody
    • Epididymis secretory sperm binding protein Li 54e antibody
    • HEL 215 antibody
    • HEL S 54e antibody
    • PHB antibody
    • PHB_HUMAN antibody
    • PHB1 antibody
    • Prohibitin antibody
    see all

画像

  • All lanes : Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771) at 1/1000 dilution

    Lane 1 : MCF-7 cell lysates
    Lane 2 : PC-12 cell lysates
    Lane 3 : A431 cell lysates
    Lane 4 : Ramos cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Prohibitin with ab75771 at 1/250.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Prohibitin with ab75711 at 1/250. An Alexa Fluor® 488-conjugated anti-rabbit IgG was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling Prohibitin with ab75771 at 1/100. A HRP/AP polymerized secondary antibody was used.

  • ab75771 (purified) at 1/500 dilution (4.3 µg/ml) immunoprecipitating Prohibitin in MCF7 whole cell lysate .
    Lane 1 (input): MCF7(Human breast adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab75771 & MCF7 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab75771 in MCF7 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM /TBST .

  • Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling Prohibitin with ab75711 at 1/250. An Alexa Fluor® 488-conjugated anti-rabbit IgG was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse endometrium tissue labelling Prohibitin with ab75771 at 1/250.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling Prohibitin with ab75771 at 1/250.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling Prohibitin with ab75771 at 1/250.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue labelling Prohibitin with ab75771 at 1/250.

参考文献

This product has been referenced in:
  • Li Y  et al. Astragaloside IV reduces neuronal apoptosis and parthanatos in ischemic injury by preserving mitochondrial hexokinase-II. Free Radic Biol Med 131:251-263 (2019). Read more (PubMed: 30502455) »
  • Kosgodage US  et al. Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells. Transl Oncol 12:513-522 (2019). Read more (PubMed: 30597288) »
See all 9 Publications for this product

レビューと Q&A

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

The immunogen sequence is from between 150-200 amino acid of PHB Swissprot 35232. The immunogen sequence does not have any homology with PHB2, Swissprot Q99623.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you very much for your patience.

I have hear back now from the laboratories in regards to the potential crossreaction of the prohibitin antibodies with T. brucei. Unfortunately none of these antibodies seems to be likely to crossreact with T. brucei. I am very sorry for this. Please find here the detailed comments:

ab115669: theexact sequence of the immunogen is proprietary. Upon alignment however with the T. brucei sequence, the homology is only 60%.
The immunogen of ab70672 it is located within aa 170-220.When comparing this immunogen sequence with the T.brucei sequence, the percentage homology is 47%.
Also for the ab75766, with an immunogen is N terminal, and ab75771, with an immunogen between in the middle, the alignment is not good enough to expect a crossreaction. Please see here the alignment of the human and T. brucei sequence http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20120213-172649-0982-54789458-pg

I am very sorry for not being able to provide you with better news. Please do let us know if there is anything else we could do to help you further.

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