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リコンビナントRabMAb

Anti-Progesterone Receptor 抗体 [SP42] (ab101688)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
  • Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP42] (ab101688)
  • Flow Cytometry - Anti-Progesterone Receptor antibody [SP42] (ab101688)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
  • Anti-Progesterone Receptor antibody [SP42] (ab101688)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP42] to Progesterone Receptor
  • Suitable for: Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-Progesterone Receptor antibody [SP42]
    Progesterone Receptor 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [SP42] to Progesterone Receptor

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt, ICC/IF, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment within Human Progesterone Receptor aa 400-550. The exact sequence is proprietary.
    Database link: P06401

  • ポジティブ･コントロール

    • IHC-P: Human breast carcinoma, Human breast, Mouse breast, Rat breastFlow Cyt: T-47D cells ICC/IF: T-47D cells

  • 特記事項

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab101688の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
1/400. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate primary antibody for 30 minutes at room temperature.
特記事項
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
1/400. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate primary antibody for 30 minutes at room temperature.

ターゲット情報

  • 機能

    The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
    Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
    Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

  • 配列類似性

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • ドメイン

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

  • 翻訳後修飾

    Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
    Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
    Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.

  • 細胞内局在

    Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • NR3C3 antibody
    • Nuclear receptor subfamily 3 group C member 3 antibody
    • PGR antibody
    • PR antibody
    • PRA antibody
    • PRB antibody
    • PRGR_HUMAN antibody
    • Progesterone receptor antibody
    • Progestin receptor form A antibody
    • Progestin receptor form B antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution (0.61 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on human breast cancer, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab101688 for 30 mins at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP42] (ab101688)
    Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP42] (ab101688)
    Immunocytochemistry/ Immunofluorescence analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab101688 at 1:100 (2.66 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry - Anti-Progesterone Receptor antibody [SP42] (ab101688)
    Flow Cytometry - Anti-Progesterone Receptor antibody [SP42] (ab101688)
    Flow Cytometry analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab101688 at 1:260 dilution (1.02 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat breast tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution (0.61 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on rat breast, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab101688 for 30 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse breast tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution (0.61 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on mouse breast, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab101688 for 30 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution (0.61 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Sporadically nuclear staining on human breast, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab101688 for 30 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
    ab101688, at a 1/400 dilution, staining Progesterone Receptor in formalin fixed, paraffin embedded Human breast carcinoma by Immunohistochemistry.
  • Anti-Progesterone Receptor antibody [SP42] (ab101688)
    Anti-Progesterone Receptor antibody [SP42] (ab101688)

参考文献 (0)

ab101688 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab101688 は論文での使用が確認できていません。

レビューと Q&A

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レビューを送る 質問を送る

Immunohistochemistry (Resin sections) abreview for Anti-Progesterone Receptor antibody [SP42]

 Excellent
Abreviews
Application
Immunohistochemistry (Resin sections)
Sample
Rat Tissue sections (Mammary Gland)
Specification
Mammary Gland
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. MdImtiazul Kabir

Verified customer

投稿 May 19 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com