Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
placenta
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Vector Mouse on Mouse (M.O.M.™ ) diluent
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Vector Mouse on Mouse (M.O.M.™ ) Kit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Abcam response
Thank you for your valuable feedback. We are sorry to see this has not worked well on this occasion.
We have offered the customer a free of charge replacement or credit note in this case.
In addition to this the following suggestions may help:
1. The antigen retrieval may require some optimization. . Consider different heating times if this has not been tried already, for example 2, 5 and 10 minutes.
2. Is the current vial of secondary antibody working well with other primary antibodies?
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Sep 01 2015