AB29473

Rat brain normal tissue lysate - membrane extract

Name: Rat brain normal tissue lysate - membrane extract (ab29473) | Abcam
Brand: Abcam Limited
SKU: AB29473
Price: 96000 JPY
Availability: InStock

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Rat brain normal tissue lysate - membrane extract suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

1 Images

Lab

Western blot - Rat brain normal tissue lysate - membrane extract (AB29473)

All lanes:

Western blot - Anti-SHANK3 antibody [N69/46] (<a href='/products/primary-antibodies/shank3-antibody-n69-46-ab193307'>ab193307</a>) at 5 µg/mL

Lane 1:

Cerebellum Mouse Tissue Lysate at 20 µg

Lane 2:

Rat Cortex at 20 µg

Lane 3:

Western blot - Rat brain normal tissue lysate - membrane extract (ab29473) at 20 µg

Lane 4:

Human Cortex at 20 µg

Lane 5:

Human Striatum Neuronal cell lysate at 20 µg

Lane 6:

Human liver tissue lysate - total protein (ab29889) at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 186 kDa

Observed band size: 186 kDa

true

Exposure time: 4min

Key facts

製品の状態

Liquid

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Samples need to boiled for 5 minutes and put on ice before use.</p>" } } }

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製品の詳細

Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

分注に関する情報

Allow to warm to room temp and agitate gently before aliquoting

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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Abcam product promise

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Rat brain normal tissue lysate - membrane extract