Nb2 nuclear extract lysate (prolactin stimulated) (ab14869)
製品の概要
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製品名
Nb2 nuclear extract lysate (prolactin stimulated)
Nb2 ライゼート 製品一覧 -
特記事項
The Nb2 nuclear extract derived from rat lymphocytes have been collected in Lysis Buffer after a 20 minute stimulation with 25 ng/ml of prolactin at 37°C immediately prior to harvesting. The protein content has been determined by a Bradford-based assay.
製品の特性
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Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading...
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研究分野
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背景
The Nb-2 rat lymphoma line was derived from a transplant of a lymphoma that developed in the thymus/lymph node of a male noble (Nb) strain rat following prolonged oestrogen treatment. The cells are of the pre-T cell origin and their proliferation is dependent on mammalian lactogens, such as prolactin. Cells have a modified prolactin receptor and are used for in vitro bioassay of lactogenic hormones, studies of the mechanisms of lactogen induced mitogenesis, studies into the action of vinca alkaloids and studies of tumour progression.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab14869 は論文での使用が確認できていません。