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AB14862

MCF7 nuclear extract lysate (H2O2 treated)

MCF7 nuclear extract lysate (H2O2 treated)

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MCF7 nuclear extract lysate (H2O2 treated). View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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Functional Studies - MCF7 nuclear extract lysate (H2O2 treated) (AB14862)
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Functional Studies - MCF7 nuclear extract lysate (H2O2 treated) (AB14862)

Each lot has been tested for p53 activation by using TransAM™ p53 Kit. The signal intensity for p53 activation in each lot is compared to the signal intensity obtained with extracts from untreated cells. Once the signals are blanked, the ratio between the signals from extracts of treated cells over untreated cells used at 5 µg/well is above 4. This ratio may vary depending on the basal level of p53 activation in a given cell type.

Key facts

細胞タイプ

MCF7

生物種

Human

組織

Breast

製品の状態

Liquid

form

疾病

Adenocarcinoma

製品の詳細

The MCF-7 nuclear extracts have been collected in Lysis Buffer after 3 hrs incubation with or without 0.2 mM hydrogen peroxide. The protein content has been determined by a Bradford-based assay.

出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle

Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Female

製品プロトコール

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