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AB14863

MCF7 nuclear extract lysate (H2O2 post treated)

MCF7 nuclear extract lysate (H2O2 post treated)

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MCF7 nuclear extract lysate (H2O2 post treated). View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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Functional Studies - MCF7 nuclear extract lysate (H2O2 post treated) (AB14863)
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Functional Studies - MCF7 nuclear extract lysate (H2O2 post treated) (AB14863)

Each lot has been tested for Sp1 activity by using the TransAM™ Sp1 Kit. The signal intensity for Sp1 activity in each lot is compared to the signal intensity obtained with H2O2-post treated extracts from MCF-7 cells. Once the signals are blanked, the ratio between the signals from untreated extracts over treated extracts used at 5 μg/well must be above 4.

Key facts

細胞タイプ

MCF7

生物種

Human

組織

Breast

製品の状態

Liquid

form

疾病

Adenocarcinoma

製品の詳細

Post-collection treatment consists in exposing the nuclear extract to a final concentration of 200 mM H2O2 for 20 min on ice. The protein content has been determined by a Bradford-based assay.

出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle

Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Female

製品プロトコール

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