MCF7 nuclear extract lysate

• WB

Lab

Western blot - MCF7 nuclear extract lysate (AB14860)

The predicted molecular weight of MPP8 is 97 kDa (SwissProt), however we expect to observe a banding pattern around 125 kDa.

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab124499 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

All lanes:

Western blot - Anti-MPP8 antibody (<a href='/products/primary-antibodies/mpp8-antibody-ab124499'>ab124499</a>) at 1 µg/mL

Lane 1:

Western blot - MCF7 nuclear extract lysate (ab14860) at 10 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 97 kDa

Observed band size: 125 kDa,80 kDa

true

Exposure time: 2min