MCF7 membrane extract lysate (ab29539)
製品の概要
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製品名
MCF7 membrane extract lysate
MCF7 ライゼート 製品一覧 -
特記事項
Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
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アプリケーション
適用あり: WBmore details
製品の特性
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Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting. -
バッファー
Constituents: 0.1% Bromophenol blue, 0.1% Magnesium chloride, 1.17% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.1% HEPES, 0.1% EDTA, 10% Glycerol, 0.1% Sodium chloride -
Concentration information loading...
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ライゼート 備考
Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. -
研究分野
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背景
MCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab29539の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.
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特記事項 |
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WB
Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab29539 は論文での使用が確認できていません。