Jurkat nuclear extract lysate (PHA stimulated) (ab14848)
製品の概要
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製品名
Jurkat nuclear extract lysate (PHA stimulated)
Jurkat ライゼート 製品一覧 -
特記事項
Cell line: Jurkat (Human T cell lymphoblast-like).
Cells were cultured in medium supplemented with 2 µg/ml PHA (Leucoagglutinin, PHA-L) for 2 days at 37°C immediately prior to harvesting.
製品の特性
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Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
バッファー
pH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading...
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研究分野
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背景
Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab14848 は論文での使用が確認できていません。