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Hep G2 nuclear extract lysate (ab14660)

Submit a review Q&A (2)References (1)

製品の概要

  • 製品名

    Hep G2 nuclear extract lysate
    HepG2 ライゼート 製品一覧

  • 特記事項

    Cell line: HepG2 (Human hepatocellular carcinoma).
    We recommend aliquoting the extracts into single use fractions and then storing them.

  • アプリケーション

    適用あり: EMSA, WBmore details

製品の特性

  • Mycoplasma free

    Yes

  • 製品の状態

    Liquid

  • 保存方法

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.90
    Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
  • Concentration information loading...

  • 研究分野

  • 背景

    Hepatitis B virus (HBV) is the major cause of acute and chronic hepatitis, leading to progressive development of necroinflammatory changes in the liver, which can result in cirrhosis and hepatocellular carcinoma. Although the development of an effective vaccine to prevent HBV infection has shown promising results and should lead to its eventual eradication, antiviral chemotherapy remains the only effective method to prevent the progression of the disease in chronic carriers. Therefore, the development of new antiretroviral agents active against HBV is needed. HepG2 cells have an epithelial morphology and are thought to be a very useful model to study HBV virus replication via transfection. Cells are also used for cancer and apoptosis studies (in particular signalling pathway studies).

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab14660の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
EMSA
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.
特記事項
EMSA
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab14660 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab14660 は 1 報の論文で使用されています。

  • Selvarajan I  et al. Integrative analysis of liver-specific non-coding regulatory SNPs associated with the risk of coronary artery disease. Am J Hum Genet 108:411-430 (2021). PubMed: 33626337

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Question

Dear Abcam,
I ordered your monoclonal Anti Tbx 3 Antibody
ab89220
and I wonder if you have positive lysate controls, for Western. In that case could you please provide me the catalogue number of these controls?
Thank you

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Answer

Thank you for your technical support and interest in our products.

As the datasheet indicates, the recommended positive control for ab89220: Anti-Tbx3 antibody is HepG2 cell lysate for Western blot application.

I have conducted a search for you and found 8 HepG2 cell lysate in our catalogue. You can use whole cell lyaste such as ab7900 or nuclear lysate (untreated) such as ab14660. Since Tbx3 is localised in the nuclear, it may be worth using the nucler fraction and enriched lysate.

I would advise you to take a look at these products to see which one would suit your need best:

https://www.abcam.com/ab7900 HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

https://www.abcam.com/ab14660: HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Question

Thanks for your letter, however you missunderstand me, most probably it is my mistake. I'd like to know how you, at Abcam, are preparing nuclear extract e.g. to produce HeLa nuclear ectract cat# 14655 or Hep G2 nuclear extract cat# 14660. How do you get rid of from cytoplams protein? Do you isolate the nucleus first, clean (wash)and than lysate them etc.? I need to know how clean are your preps before I can order your produts # 14655 or # 14660.

Read More
Answer

Thank you for your enquiry. The nuclear extracts were prepared as detailed in: Dignam, J.D., Lebovitz, R.M. and Roeder, R.G. (1983) Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucl. Acids Res. 11, 1475–89. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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