AB14653

HeLa nuclear extract lysate (TPA stimulated 2 hr)

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HeLa nuclear extract lysate (TPA stimulated 2 hr) suitable for EMSA, WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

2 Images

Project5632****

Western blot - HeLa nuclear extract lysate (TPA stimulated 2 hr) (AB14653)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab55160).

All lanes:

Western blot - Anti-Egr1 antibody [mAbcam55160] - BSA and Azide free (<a href='/products/primary-antibodies/egr1-antibody-mabcam55160-bsa-and-azide-free-ab264527'>ab264527</a>) at 5 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

Western blot - HeLa nuclear extract lysate (TPA stimulated 2 hr) (ab14653) at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 57 kDa

Observed band size: 55 kDa,85 kDa

false

Project5632****

Western blot - HeLa nuclear extract lysate (TPA stimulated 2 hr) (AB14653)

All lanes:

Western blot - Anti-Egr1 antibody [mAbcam55160] (<a href='/products/primary-antibodies/egr1-antibody-mabcam55160-ab55160'>ab55160</a>) at 5 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

Western blot - HeLa nuclear extract lysate (TPA stimulated 2 hr) (ab14653) at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 57 kDa

Observed band size: 55 kDa,85 kDa

false

Key facts

細胞タイプ

HeLa

生物種

Human

組織

Cervix

製品の状態

Liquid

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疾病

Adenocarcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "EMSA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Cells were cultured in medium supplemented with 50 ng/mL TPA (phorbol, 12 myristate, 13 acetate) for two hours at 37°C immediately prior to harvesting. We recommend aliquoting the extracts into single use fractions and then storing.

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

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HeLa nuclear extract lysate (TPA stimulated 2 hr)