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A-431 nuclear extract lysate (EGF treated) (ab14635)

Submit a review Q&A (2)

製品の概要

  • 製品名

    A-431 nuclear extract lysate (EGF treated)
    A431 ライゼート 製品一覧

  • 特記事項

    Cell line: A431 (Human epithelial carcinoma).
    Cells were cultured in medium supplemented with 10 ng/mL EGF (epidermal growth factor) for 10 minutes at 37°C immediately prior to harvesting.
    We recommend aliquoting the extracts into single use fractions and then storing.

製品の特性

  • Mycoplasma free

    Yes

  • 製品の状態

    Liquid

  • 保存方法

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.90
    Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
  • Concentration information loading...

  • 研究分野

  • 背景

    A431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab14635 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab14635 は論文での使用が確認できていません。

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Question

Oui d’accord, j’apprécierais un lysat d’une des deux lignées, n’importe laquelle. Ce serait donc un bon contrôle pour stat5 total et phosphorylé?

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Answer

J'ai mis en place l'envoi d'une unité gratuite de lysat de A431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treated, référence ab14635. Le numéro de commande est : *****.

Ce lysat est recommandé comme contrôle positif pour STAT5 phosphorylé et devrait convenir également pour l'anti-STAT5 ab7969.

J'espère que ce lysat vous sera utile. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Question

What antibody you do QC in this nuclear lysate?

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Answer

Thank you for your enquiry. ab14635 (A431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treated) has been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). Positive Transcription Factors in Western: c-Jun, C/EBPb, Sp1, YY1 and in EMSA: Sp1, USF, YY1. Please don't hesitate to contact us again if you have further questions.

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