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AB234617

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

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Prestained Protein Ladder ab234617 is a blue protein standard with 11 pre-stained proteins covering a range of MW from 10-180 kDa.
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SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB234617)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB234617)

SDS-PAGE analysis with ab234617.

Gel 1 : Tris-Glycine 10%

Gel 2 : Bis-Tris 4-12% MOPS buffer

Gel 3 : Bis-Tris 4-12% MES buffer.

The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis conditions.

Key facts

アプリケーション

SDS-PAGE, WB

applications

製品の状態

Liquid

form

バッファー組成

pH: 7.5 Constituents: 21.6% Urea, 15% Glycerol (glycerin, glycerine), 2% Sodium lauryl sulfate, 0.44% Tris phosphate, 0.003% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 3-5 µl per well for 15-well or 10-well mini-gel, respectively.</p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 1.5-2.5 μl.</p>" } } }

製品の詳細

Prestained Protein Ladder ab234617 is a blue protein standard with 11 pre-stained proteins covering a range of MW from 10-180 kDa. ab234617 is designed for monitoring protein seperation in SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Key product features:

  • Broad range: 10-180 kDa
  • Ready to use: The protein ladder is supplied in the gel loading buffer and is ready to use.
  • Easily identified: Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-glycine buffer).

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 1.5-2.5 μl per well for general Western transferring.

Review other protein ladders in the unstained and prestained protein ladder guide.

出荷温度及び保存条件

出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C

製品プロトコール

ターゲットの情報

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