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AB116027

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

5

(2 Reviews)

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(5 Publications)

10-180 kDa prestained protein ladder (ab116027) is a ready- to-use, three-color protein standard with 10 pre-stained proteins ranging from molecular weights 10 to 180 kDa.

- Suitable for western blot (WB) and SDS-PAGE
- Suitable for Tris Glycine (4-12%) SDS buffer, Bis-Tris (4-12%) MOPS buffer and Bis-Tris (4-12%) MES buffer
- Part of our Lab essential range western blot workflow
2 Images
SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB116027)
  • SDS-PAGE

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SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB116027)

Gel 1 : Tris-Glycine (~4-20%) Gel 2 : Bis-Tris (12%) MOPS buffer Gel 3 : Bis-Tris (10%) MES buffer Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.

Western blot - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB116027)
  • WB

AbReview47271****

Western blot - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB116027)

Very easy to use protein ladder in the range from 10-180 kDa. Good separation in SDS-PAGE and nice transfer onto nitrocellulose membrane. The gel used was a 10% Tris-Bis gel from life-technologies. the protein ladder was used at 2.5μl per well.

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This image is courtesy of an anonymous Abreview.

Key facts

アプリケーション

SDS-PAGE, WB

applications

製品の状態

Liquid

form

バッファー組成

pH: 7.5 Constituents: 15% Glycerol (glycerin, glycerine), 3% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 2% Sodium lauryl sulfate, 1% Tris phosphate, 0.02% Urea

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組合せ製品

Reagents, Controls & Accessories

AB116028

Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (AB116028)

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  • 3
Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

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  • 1
Reagents, Controls & Accessories

AB234592

Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)

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  • 0
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.</p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>~2 - 3 µl per well for general Western transfering. Apply more for thicker (&gt;1.5 mm) or larger gel.</p>" } } }
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製品の詳細

Prestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).

This product was previously called Prism Ultra Protein Ladder (10 - 180 kDa).

This prestained protein ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use.

Do not heat, dilute, add reducing agent before loading.

Key product features:

  • Broad range: 10-180 kDa
  • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
  • Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
  • Sharp bands
  • Recommended loading: ~2 - 3 μl for western blots

Review other protein ladders in the unstained and prestained protein ladder guide.

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
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製品プロトコール

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ターゲットの情報

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

BMC infectious diseases 21:300 PubMed33761869

2021

Isolation and characterizations of a novel recombinant scFv antibody against exotoxin A of Pseudomonas aeruginosa.

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Zahra Shadman,Safar Farajnia,Mohammad Pazhang,Mohammadreza Tohidkia,Leila Rahbarnia,Saeed Najavand,Sayna Toraby

Saudi journal of biological sciences 28:891-900 PubMed33424381

2021

Effect of sulfur-containing agrochemicals on growth, yield, and protein content of soybeans (Glycine max (L.) Merr).

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Mukhambetkali Burkitbayev,Nadezhda Bachilova,Meruyert Kurmanbayeva,Karakoz Tolenova,Nurgul Yerezhepova,Moldir Zhumagul,Asem Mamurova,Bexultan Turysbek,Gulim Demeu

Journal of cell communication and signaling 15:107-123 PubMed33398722

2021

Lactate regulates autophagy through ROS-mediated activation of ERK1/2/m-TOR/p-70S6K pathway in skeletal muscle.

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Rohollah Nikooie,Daruosh Moflehi,Samira Zand

Cell & bioscience 7:71 PubMed29270287

2017

Hypoxic 3D in vitro culture models reveal distinct resistance processes to TKIs in renal cancer cells.

Applications

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Zofia F Bielecka,Agata Malinowska,Klaudia K Brodaczewska,Aleksandra Klemba,Claudine Kieda,Paweł Krasowski,Elżbieta Grzesiuk,Jan Piwowarski,Anna M Czarnecka,Cezary Szczylik

Journal of cell science 127:124-36 PubMed24144701

2013

Entry into the nuclear pore complex is controlled by a cytoplasmic exclusion zone containing dynamic GLFG-repeat nucleoporin domains.

Applications

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Jindriska Fiserova,Matthew Spink,Shane A Richards,Christopher Saunter,Martin W Goldberg
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
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