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AB234592

Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)

Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)

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(1 Publication)

6.5-270 kDa prestained protein ladder (ab234592) is a ready-to-use, three-color protein standard with 10 pre-stained proteins ranging from molecular weights 6.5 to 270 kDa.

- Suitable for western blot (WB) and SDS-PAGE
- Suitable for Tris Glycine (4-12%) SDS buffer, Bis-Tris (4-12%) MOPS buffer and Bis-Tris (4-12%) MES buffer
- Part of our Lab essentials range western blot workflow
1 Images
SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)

SDS-PAGE analysis with ab234592.

Gel 1 : Tris-Glycine 4-20%

Gel 2 : Bis-Tris 4-12% MES buffer.

The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.

Key facts

アプリケーション

SDS-PAGE, WB

applications

製品の状態

Liquid

form

バッファー組成

pH: 7.5 Preservative: 0.01% Sodium azide Constituents: 33% Glycerol (glycerin, glycerine), 2% Sodium lauryl sulfate, 1.37% Tris phosphate, 0.15% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.03% EDTA

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 3-5 µl per well for 15-well or 10-well mini-gel, respectively.</p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 2.5 μl.</p>" } } }
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製品の詳細

Prestained Protein Ladder ab234592 is a three-color protein standard with 10 pre-stained proteins covering a range of MW from 6.5-270 kDa. This prestained protein ladder allows you to monitor protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Key product features:

  • Broad range: 6.5-270 kDa
  • Ready to use: The ladder is supplied in the gel loading buffer and is ready to use.
  • Easily identified: Proteins are covalently coupled with a blue chromophore except three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa).

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Review other protein ladders in the unstained and prestained protein ladder guide.

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
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製品プロトコール

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ターゲットの情報

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 10:14724 PubMed32895418

2020

Oncogenes overexpressed in metastatic oral cancers from patients with pain: potential pain mediators released in exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Aditi Bhattacharya,Malvin N Janal,Ratna Veeramachaneni,Igor Dolgalev,Zinaida Dubeykovskaya,Nguyen Huu Tu,Hyesung Kim,Susanna Zhang,Angie K Wu,Mari Hagiwara,A Ross Kerr,Mark D DeLacure,Brian L Schmidt,Donna G Albertson
View all publications
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組合せ製品

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Reagents, Controls & Accessories

AB116029

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SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

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Reagents, Controls & Accessories

AB116027

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SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB116027)

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Abcam product promise

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