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AB313996

Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)

Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)

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Prestained Protein Ladder AB313996 is a combination of 11 pre-stained proteins with molecular weights from 3 to 260 kDa. This prestained protein ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
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SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (AB313996)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (AB313996)

Guide for Molecular Weight Estimation (kDa)

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Note : The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

Key facts

アプリケーション

SDS-PAGE, WB

applications

製品の状態

Liquid

form

バッファー組成

pH: 7 - 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 25% Urea, 3% Sodium lauryl sulfate, 2% Tris phosphate, 0.016% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 3-5 µl per well for 15-well or 10-well mini-gel, respectively.</p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 2.5 μl.</p>" } } }

製品の詳細

Prestained Protein Ladder ab313996 is a combination of 11 pre-stained proteins with molecular weights from 3 to 260 kDa. This prestained protein ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
Key Product Features: Extra broad molecular weight (3-260 kDa).
Ready to use: The 11 recombinant proteins are covalently coupled with blue chromophore, while 2 red bands at 70 kDa and 260 kDa, a green band at 15 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

出荷温度及び保存条件

出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C

製品プロトコール

ターゲットの情報

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