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AB325997

Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color)

Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color)

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Prestained Protein Ladder ab325997 is a combination of 13 pre-stained proteins covering a range of MW from 5-250 kDa.
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SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color) (AB325997)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color) (AB325997)

The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

Key facts

アプリケーション

SDS-PAGE, WB

applications

製品の状態

Liquid

form

バッファー組成

pH: 7.5 Constituents: 27.9% Glycerol (glycerin, glycerine), 25% Urea, 14.7% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 2.7% Sodium lauryl sulfate, 2% Tris phosphate

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.</p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.</p>" } } }

製品の詳細

Prestained Protein Ladder ab325997 is a combination of 13 pre-stained proteins covering a range of MW from 5-250 kDa. It is designed for monitoring protein seperation in SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Key product features:

  • Broad range: 5-250 kDa
  • Ready to use: The protein ladder is supplied in the gel loading buffer and is ready to use.
  • Easily identified: Proteins are covalently coupled with a blue chromophore, while a red band at 80 kDa, a green band at 25 kDa, a newly designed yellow band at 15 kDa and a purple band at 250 kDa serve as reference bands.

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Review other protein ladders in the unstained and prestained protein ladder guide.

出荷温度及び保存条件

出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C

製品プロトコール

ターゲットの情報

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