Native lysis Buffer (ab156035)
製品の概要
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製品名
Native lysis Buffer
Lysis Buffer ihc 試薬 製品一覧 -
アプリケーション
適用あり: WB, Sandwich ELISA, SDS-PAGE, IPmore details -
特記事項
Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells. This reagent extracts cytoplasmic, nuclear and membrane proteins. The detergent used in this buffer is relatively gentle and in many cases allows solubilization of protein complexes that retain enzymatic activity. On the flip side, the gentle nature of this lysis buffer means that it may result in extractions that are less complete than lysis buffers using more stringent detergents (e.g. ab156034). Native lysis buffer is compatible with many downstream applications, including enzyme activity assays, SDS-PAGE, Western blot, immunoprecipitation, ELISA and BCA assays.
製品の特性
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製品の状態
Liquid -
保存方法
Store at +4°C. -
バッファー
pH: 7.50
Constituents: 0.02% Beta glycerophosphate, 0.02% Sodium orthovanadate, 0.04% EGTA, 0.6% HEPES, 0.03% EDTA, 0.58% Sodium chloride, 1.5% Dodecyl maltoside, 0.11% Sodium pyrophosphate decahydrate -
Concentration information loading... -
研究分野
関連製品
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Related Buffer
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アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab156035の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| Sandwich ELISA |
Use at an assay dependent concentration.
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| SDS-PAGE |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. |
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Sandwich ELISA
Use at an assay dependent concentration. |
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SDS-PAGE
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
画像
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SDS-PAGE - Native lysis Buffer (ab156035)HeLa cell extraction using ab156035.
2.5 million HeLa cells were lysed on ice for 15 minutes with 0.5 mL of 1X ab156035. Next the sample was centrifuged at 14,000 rpm at 4ºC for 15 minutes: the supernatant ( = cleared lysate) was removed and the pellet ( = insoluble material) was resuspended in 0.5 mL lysis buffer and solubilized by sonication. Equivalent loads of the cleared lysate and solubilized pellet were analyzed by SDS-PAGE and Coomassie stain.
BCA protein concentration determination of the soluble and insoluble material indicates that a total of 1.15mg of protein was recovered and 70% was in the soluble cleared cell lysate.Lane 1: MW marker
Lane 2: Cleared lysateLane 3: Non-soluble
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (4)
ab156035 は 4 報の論文で使用されています。
- Gupta U et al. Increased LCN2 (lipocalin 2) in the RPE decreases autophagy and activates inflammasome-ferroptosis processes in a mouse model of dry AMD. Autophagy 19:92-111 (2023). PubMed: 35473441
- Wu J et al. Exploring dynamic change and influencing factors of TGF-β1 and MUC1 in human breast milk, and their association with infantile diseases. Transl Pediatr 12:1139-1147 (2023). PubMed: 37427064
- He D et al. Methionine oxidation activates pyruvate kinase M2 to promote pancreatic cancer metastasis. Mol Cell 82:3045-3060.e11 (2022). PubMed: 35752173
- Zhai L & Wang X Syringaresinol-di-O-ß-D-glucoside, a phenolic compound from Polygonatum sibiricum, exhibits an antidiabetic and antioxidative effect on a streptozotocin-induced mouse model of diabetes. Mol Med Rep 18:5511-5519 (2018). PubMed: 30365054
