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Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)

Submit a review Q&A (1)References (25)
IHC - Wholemount - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)
  • Western blot - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)

Key features and details

  • Sheep Anti-Rabbit IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Sheep
  • Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr

Conjugates logo Related conjugates and formulations

DyLight® 488 DyLight® 650 FITC Texas Red ® TRITC Unconjugated

製品の概要

  • 製品名

    Sheep Anti-Rabbit IgG H&L (HRP)
    IgG 二次抗体 製品一覧

  • 由来種

    Sheep

  • ターゲット生物種

    Rabbit

  • アプリケーション

    適用あり: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details

  • 免疫原

    Rabbit IgG, whole molecule

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 6.5
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose.

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6795の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/2500.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/2500.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

画像

  • IHC - Wholemount - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)
    IHC - Wholemount - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)This image is courtesy of an Abreview submitted by Michal Szlis
  • Western blot - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)
    Western blot - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)

    ab6795 was used at dilution 1/2000 with the primary antibody ab8895 in WB. See the review on ab8895.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (25)

ab6795 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6795 は 25 報の論文で使用されています。

  • Wang B  et al. Bigelovin inhibits hepatocellular carcinoma cell growth and metastasis by regulating the MAPT-mediated Fas/FasL pathway. Neoplasma 70:208-215 (2023). PubMed: 36812233
  • Wang T  et al. LncAABR07053481 inhibits bone marrow mesenchymal stem cell apoptosis and promotes repair following steroid-induced avascular necrosis. Commun Biol 6:365 (2023). PubMed: 37012358
  • Du H  et al. Polyphyllin I attenuates the invasion and metastasis via downregulating GRP78 in drug-resistant hepatocellular carcinoma cells. Aging (Albany NY) 15:12251-12263 (2023). PubMed: 37934581
  • Afewerky HK  et al. Sodium-calcium exchanger isoform-3 targeted Withania somnifera (L.) Dunal therapeutic intervention ameliorates cognition in the 5xFAD mouse model of Alzheimer's disease. Sci Rep 12:1537 (2022). PubMed: 35087161
  • Peng L  et al. Transcription factor SNAI2 exerts pro-tumorigenic effects on glioma stem cells via PHLPP2-mediated Akt pathway. Cell Death Dis 13:516 (2022). PubMed: 35654777
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

Question

Hi Abcam,

I tried contacting the abcam distributer here in Australia (Sapphire bioscience), but the representative was unable to answer my questions.

I would like to purchase one secondary antibody against primary abcam antibodies I already have.

I would like to use the antibodies in a dot blot assay, using the ECL detection method.

There are three possible secondary antibodies that seem to me like they will work for two of my primaries:

Primary: ab8898 and ab9051
Secondary: ab97064, ab97095 and ab97200

Which secondary should I choose that will be best for both of my primaries, using ECL detection in a Dot Blot assay?

Thank you in advance for your assistance.

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Answer

Thank you for contacting us.

The secondary antibodies you mentioned might work for dot blots with the rabbit polyclonal Histone primaries, as they are guaranteed to work in Western blot which is quite similar to dot blot. However, we have not tested them specifically for this application and thus cannot guarantee that it would work.

Therefore, I would like to recommend one of the following secondaries which have been tested in dot blot:

ab6721: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-ab6721.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-ab6721.html).

ab6802: https://www.abcam.com/Donkey-Rabbit-IgG-HL-HRP-ab6802.html (or use the following: https://www.abcam.com/Donkey-Rabbit-IgG-HL-HRP-ab6802.html).

ab6795: https://www.abcam.com/Sheep-Rabbit-IgG-HL-HRP-ab6795.html (or use the following: https://www.abcam.com/Sheep-Rabbit-IgG-HL-HRP-ab6795.html).

They are raised in goat, donkey and sheep and apart from this are equally well suited for your experimental setup. If you need a decision guiding you could for example choose the one with the most references: this would be ab6721 with 52 publications mentioning the use of this antibody.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (https://www.abcam.com/Abpromise).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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